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Updated: Jul 20, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

来自老年男性的精子中的圆形RNA的表达特征

Qiao Zhou¹, Anming Liu¹, Hui Ji¹

¹Department of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital, Women's Hospital of Nanjing Medical University, Nanjing, 210004, China.

Molecular biology reports

|August 4, 2023

概括

先进的父亲年龄通过精子中改变的循环RNA (circRNA) 表达方式影响生育能力. 这项研究确定了特定的circRNA和受影响的途径,为男性生殖衰老机制提供了洞察力.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

先进的父亲年龄 (APA) 与降低生育能力有关,但其潜在机制尚不清楚.
循环RNAs (circRNAs) 涉及精子生成和疾病生物标志物应用.
研究老年男性精子中的circRNA概况对于了解生育能力下降至关重要.

研究的目的:

为了探索老年男性的精子中的circRNA表达特征.
为了确定与晚年父亲年龄相关的差异表达的circRNAs (DE-circRNAs).
阐明DE-circRNAs在衰老的雄性性交配体中影响的功能途径.

主要方法:

从年轻 (<40岁) 和年长 (≥40岁) 的男性收集精子.
RNA测序以描述circRNA表达的情况.
RT-qPCR用于验证DE-circRNAs. 的验证.
生物信息分析 (KEGG,基因本体学) 用于功能途径评估.

主要成果:

总共有18787个环RNA被确定.
在晚年父亲年龄组中,有1056个circRNAs被下调和1228个被上调.
受影响的途径包括DNA修复,介质重组和PI3K/AKT信号传递.

结论:

circRNAs在老年男性的精子中发挥着重要作用.
这项研究为精子衰老的调节机制提供了新的见解.
circRNA表达特征可以作为生物标志物来评估与年龄相关的男性生殖健康.

关键词:

老龄化的男人老龄化循环RNAs 是一个循环RNA.这就是ROSOS ROS.精子动物 (Spermatozoa) 是一种精子.

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