循环RNAcirc_0000119通过miR-433-3p/PAK2轴促进宫癌细胞的生长和迁移
Junxiao Zhang1, Guanghua Chu2, Lihua Zheng3
1Third Department of Gynecology, Northwest Women's and Children's Hospital, No. 1616, Yanxiang Road, Yanta District, Xi'an, 710061, People's Republic of China. fakcdyx@163.com.
Journal of applied genetics
|August 4, 2023
概括
这项研究表明,高的circ_0000119促进结直肠癌 (CC) 的进展. 击败circ_0000119通过miR-433-3p/PAK2途径抑制CC细胞生长和转移,提供潜在的治疗标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 结肠直肠癌 (CC) 仍然是一个重要的健康问题,具有复杂的分子基础.
- 循环RNAs (circRNAs) 正在成为各种癌症的关键调节者,包括CC.
- 了解circ_0000119等circRNAs的特定作用对于开发新型治疗策略至关重要.
研究的目的:
- 为了研究circ_0000119在结直肠癌 (CC) 进展中的作用.
- 阐明circ_0000119在CC中的功能背后的分子机制.
- 为了探索针对CC处理的circ_0000119/miR-433-3p/PAK2轴的潜力.
主要方法:
- 定量实时聚合酶链反应 (qRT-PCR) 用于测量表达水平.
- 细胞检测包括MTT,EdU,殖民地形成,流细胞计和Transwell检测,以评估增殖,细胞周期,细胞亡,迁移和入侵.
- 生物信息学预测,双 luciferase 记者基因测试,RNA 免疫沉 (RIP) 和RNA 拉下测试以确认分子相互作用.
- 在体内异种移植模型和免疫组织化学 (IHC) 用于评估瘤生长和Ki67表达.
主要成果:
- 在CC组织和细胞系中,Circ_0000119被显著上调.
- 抑制circ_0000119抑制了CC细胞的增殖,迁移,入侵和细胞周期进展,同时促进了细胞亡.
- 大约有0000119个海绵式miR-433-3p,miR-433-3p的向是p21激活激酶2 (PAK2).
- Circ_0000119 knockdown 在体内抑制了瘤生长.
结论:
- 在结直肠癌中,circ_0000119作为一种致癌性circRNA.
- 环_0000119/miR-433-3p/PAK2轴在调节CC进展方面发挥着至关重要的作用.
- Circ_0000119是抑制结直肠癌发展的潜在治疗标.
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