三倍三倍的消灭升级基于转换的氧气传感器来克服自发光的局限性
Adrian A Mendonsa1, Cassandra C Soeldner1, Natalie E Mudd2
1Chemical and Biological Engineering, Colorado School of Mines, Golden, Colorado 80401, United States.
ACS sensors
|August 4, 2023
概括
这项研究引入了一种新的纳米传感器,利用向上转换来测量生物系统中溶解的氧气. 这项技术有效地克服了自光干扰,使酵母中的代谢活动能够得到更清晰的监测.
科学领域:
- 生物成像和纳米传感器技术
- 生物物理化学 生物物理化学
- 代谢活动监测 代谢活动监测
背景情况:
- 生物成像中的自发光构成了一个重大挑战,它会掩盖来自光探针和标记器的信号.
- 向上转换提供了一种潜在的解决方案,可以克服生物成像中的自流光限制.
- 精确监测溶解氧气对于了解细胞代谢过程至关重要.
研究的目的:
- 开发一种能够光学报告溶解氧度变化的纳米传感器.
- 为了利用三倍三倍的消灭升级转换来克服生物成像中的自流体.
- 通过跟踪氧气消耗来监测Saccharomyces cerevisiae中的代谢活动.
主要方法:
- 一个纳米传感器的开发基于三倍三倍的消灭上转换.
- 使用一种敏感剂-消灭剂染料对: (II) 八甲基和9,10-二乙.
- 随着时间的推移,监测Saccharomyces cerevisiae中的氧气消耗.
主要成果:
- 纳米传感器对0.00至3.17毫克/升O2.2的溶解氧水平表现出敏感性.
- 传感器在多个周期中表现出良好的可逆性,并保持了7天的信号和体稳定性.
- 在纳米传感器发射和酵母自身光之间没有观察到信号重叠.
结论:
- 向上转换提供了一种简单且经济的方法,可以在生物成像中克服自身光.
- 开发的纳米传感器有效监测生物系统中溶解氧和代谢活动.
- 这项技术对需要增强信号检测的先进生物成像应用具有前景.
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