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相关概念视频

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

10.7K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.1K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.8K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

81
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
81
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

11.5K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
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相关实验视频

Updated: Jul 20, 2025

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
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Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

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免疫光显微镜 显微镜

Domenico F Galati1, David J Asai2

  • 1Biology Department, College of Science and Engineering, Western Washington University, Bellingham, Washington.

Current protocols
|August 4, 2023
PubMed
概括
此摘要是机器生成的。

免疫光显微镜可视化细胞分子使用光抗体. 本指南详细介绍了各种细胞类型的协议和先进的成像技术,包括超分辨率显微镜.

关键词:
泰特拉希米纳是什么意思细胞骨架 细胞骨架光是一种光.免疫光效应 免疫光效应微管是微管的组成部分.氨酸抗体的抗体

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Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
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Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

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相关实验视频

Last Updated: Jul 20, 2025

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
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Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

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Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
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Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

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科学领域:

  • 细胞生物学 细胞生物学
  • 显微镜的使用方法
  • 分子生物学分子生物学

背景情况:

  • 可视化光标记的分子揭示了细胞动态.
  • 免疫光显微镜使用光标记的抗体来检测特定的分子.
  • 有效的应用需要仔细考虑抗原,抗体,固定和成像.

研究的目的:

  • 为免疫光染色和成像提供详细的协议.
  • 覆盖多种细胞类型,包括附着纤维细胞和悬浮Tetrahymena.
  • 为了使用广场,共聚焦和超分辨率显微镜实现可视化.

主要方法:

  • 附着细胞 (纤维细胞) 的免疫光染色方案.
  • 悬浮细胞的免疫光染色协议 (Tetrahymena).
  • 使用广场,激光扫描共聚焦和超分辨率 (SRRF) 显微镜的可视化协议.

主要成果:

  • 在纤维细胞和四膜中成功进行免疫光染色的证明方案.
  • 能够可视化不同细胞类型的细胞骨架和细胞器.
  • 通过一系列光显微镜技术促进成像.

结论:

  • 免疫光显微镜是一种用于可视化细胞结构的多功能技术.
  • 标准化协议可以适应各种细胞类型和先进的成像.
  • 这项工作为使用免疫光显微镜的研究人员提供了全面的资源.