Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Isolation01:24

DNA Isolation

39.4K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
39.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Targeted extracellular degradation of LRP8 promotes ferroptosis in cancer cells.

bioRxiv : the preprint server for biology·2026
Same author

Autophagolysosomal exocytosis inverts Src kinase onto the cell surface in cancer.

Science (New York, N.Y.)·2026
Same author

Directed Evolution of Enzymes for Bioorthogonal Chemistry Using Acid Chloride Proximity Labeling.

ACS central science·2026
Same author

Targeted shedding of extracellular membrane proteins by induced protease recruitment.

bioRxiv : the preprint server for biology·2026
Same author

Primate lineage specification requires suppression of Alu hyperediting.

bioRxiv : the preprint server for biology·2026
Same author

Regarding Emitter Positioning for Nanoflow Electrospray Ionization with a High-Capacity Inlet Capillary.

Journal of the American Society for Mass Spectrometry·2026

相关实验视频

Updated: Jul 20, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

18.2K

用于DNA编码图书馆的DNA兼容铜/TEMPO氧化

Justice L Merrifield1, Edward B Pimentel1, Trenton M Peters-Clarke1,2

  • 1Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.

Bioconjugate chemistry
|August 4, 2023
PubMed
概括

研究人员开发了一种与DNA兼容的方法,使用铜/TEMPO催化剂将酒精氧化为化物. 这一进步有助于DNA编码图书馆的建设和多样化.

更多相关视频

Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

3.5K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

10.4K

相关实验视频

Last Updated: Jul 20, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

18.2K
Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

3.5K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

10.4K

科学领域:

  • 有机化学 有机化学
  • 药用化学 医学化学
  • 生物技术是生物技术.

背景情况:

  • 化物是DNA编码库 (DEL) 构建中的关键构建块.
  • 在DEL化学中,开发DNA兼容的方法来将酒精氧化为化物是一个重大挑战.

研究的目的:

  • 开发一种溶液相,DNA兼容的方法,用于将DNA连接的初级酒精氧化为化物.
  • 为了证明得到的化物对图书馆多样化的有用性.

主要方法:

  • 使用铜/TEMPO催化剂系统进行氧化.
  • 在半水态,室温条件下进行反应.
  • 使用质谱,qPCR,桑格测序和结合试验验证了DNA兼容性.

主要成果:

  • 在基醇,异基醇和基醇的氧化中获得高产量.
  • 通过多种分析技术证实了DNA后反应的完整性.
  • 证明了生成的化物的成功后续转化.

结论:

  • 开发的铜/TEMPO催化氧化是一种强大且与DNA兼容的生成化物的方法.
  • 这种方法通过使图书馆实现高效和多功能多样化,显著提升了DEL构建.