形和形相似基因对于大米的叶子形态发生是必需的
Jiajun Liu1,2, Xi'an Shi1, Tianhui Zhong1
1College of Agriculture, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, People's Republic of China.
Plant & cell physiology
|August 4, 2023
概括
辅助运输对于叶的发育至关重要. OsPID,OsPIN1c和OsPIN1d基因的突变导致叶子形状和融合的严重缺陷,揭示了它们的基本作用.
科学领域:
- 植物生物学 植物生物学
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 素是调节植物生长和发育的关键激素,特别是叶子形态发生.
- 米 (Oryza sativa L.) 叶子发育中的辅酶运输蛋白的特定功能尚不清楚.
研究的目的:
- 研究PINOID (OsPID) 和两个PIN1姐妹蛋白 (OsPIN1c和OsPIN1d) 在叶发育中的作用.
- 在单种植物模型中阐明奥素运输对叶子形态发生的贡献.
主要方法:
- 在大米中生成和分析OsPID,OsPIN1c和OsPIN1d的零突变线.
- 单一和三重突变的叶子形态的表型特征.
- 对OsPIN1c和OsPID的亚细胞局部化研究.
- RNA测序用于分析突变物中的基因表达变化.
主要成果:
- 在OsPIN1c和OsPIN1d中功能丧失的突变体表现出严重的叶子缺陷,包括垂落的叶片,加厚的关节和缺乏和耳朵的合叶子.
- OsPID突变体表现出类似但不那么严重的缺陷,特别是缺乏叶子融合.
- 突变往往导致叶子的双侧叶子形.
- OsPIN1c和OsPID被局部化到启动叶子原始体.
- 三重突变体表现出更严重的器官生成缺陷,关键叶子发育基因的表达发生显著改变.
结论:
- OsPID,OsPIN1c和OsPIN1d对于水叶的正常发育和形态发生是必不可少的.
- 这些辅酶运输成分在调节叶片叶片形状,膜-膜关节形成和叶片融合方面发挥着至关重要的作用.
- 这项研究强调了辅酶运输在不同植物物种的叶子发育中的保留和特定作用.
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