关于Bombyx mori (Lepidoptera: Bombycidae) 的功能性表征C型讲素 5 5
Yan-Xia Sun1,2, Bang-Xian Zhang1,2,3, Fang-Fang Liu1,2
1Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China.
Journal of economic entomology
|August 4, 2023
概括
丝虫C型讲蛋白5 (CTL5) 通过结合血细胞和刺激免疫基因表达来增强病原体的清除. 这种蛋白质充当松素,增强Bombyx mori.的细胞和幽默性先天免疫力.
科学领域:
- 昆虫免疫学 昆虫免疫学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- C型讲蛋白 (CTLs) 是模式识别受体 (PRRs),对于调节免疫反应至关重要.
- 之前已经观察到丝虫Bombyx mori CTL5蛋白在细菌清除中的作用,但其机制仍然不清楚.
研究的目的:
- 阐明Bombyx mori CTL5 (BmCTL5) 对天生的免疫有所贡献的机制.
- 研究BmCTL5.5的表达模式,结合性质和免疫调节功能.
主要方法:
- 在不同发育阶段和组织中分析BmCTL5基因表达.
- 重组蛋白表达和与血细胞,微生物成分和整合素子单元的结合测试.
- 评估BmCTL5对血细胞细胞分裂和免疫基因表达的影响.
- 转录组测序和BmCTL5刺激血细胞的功能丰富分析.
主要成果:
- BmCTL5主要表达在幼虫和成年中,对病原体和20-hydroxyecdysone的反应不同.
- 重组BmCTL5蛋白与血细胞,微生物多糖,细菌和Beauveria bassiana子结合,并与整合素β2-β5子单元相互作用.
- BmCTL5增强了血细胞细胞分裂,并刺激了抗菌和免疫信号分子的表达.
- 转录组分析显示,在BmCTL5刺激时,先天免疫反应和信号通路的显著丰富.
结论:
- BmCTL5作为一种素,增强病原体清除的功能.
- 它调节丝虫的幽默性 (免疫基因表达) 和细胞性 (细胞) 免疫反应.
- 这项研究提供了关于昆虫天生的免疫中CTLs分子机制的见解.
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