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优格莱娜:由氧酸盐复合物和不同的热量条件调节的膜结合式外酶活性的生物化学特性

Anita Leocadio Freitas-Mesquita¹, Luiz Fernando Carvalho-Kelly¹, Thaís Souza Silveira Majerowicz²

¹Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, RJ, Brazil; Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagem, 21941-590 Rio de Janeiro, RJ, Brazil.

European journal of protistology

|August 4, 2023

概括

这项研究在生化学上描述了Euglena gracilis中的ecto-phosphatase活性,发现它是酸性,铜刺激,并被各种离子抑制. 这些见解有助于理解它的生态作用.

科学领域:

生物化学生物化学
酶学是一种酶学.
微生物学微生物学

背景情况:

超酸酶是细胞表面酶,具有细胞外催化场所.
Euglena gracilis是一种典型的淡水微藻,以其无处不在和抗压力而闻名.

研究的目的:

为了生化地描述Euglena gracilis的外酸酶活性.
确定这种酶活性的动力参数并确定其调节器.

主要方法:

生物化学测试以测量ecto-酸酶活性.
使用p-nitrophenyl phosphate (p-NPP) 作为基质进行酶动力学分析.
测试各种离子 (铜,,酸盐,化物) 和抑制剂 (orthovanadate,无机酸盐,酸复合物) 的作用.

主要成果:

酸酸酶活性表现出酸性pH最佳值,并受到铜的刺激.
表面运动参数:K_m = 2.52 ± 0.12 mM p-NPP,V_max = 3.62 ± 0.06 nmol p-NP × h^-1 × 10⁶ 细胞.
,奥托瓦纳酸盐,酸盐,化物,无机酸盐和酸复合物抑制了活性.
在培养生长过程中,活性保持不变,但随着黑暗诱导的代谢变化而下降.

结论:

在E. gracilis中特征化的ecto-phosphatase活性具有特定的动力和调节性质.
氧酸复合物被确定为自然环境中的潜在抑制剂.
了解这些参数对于阐明酶在E. gracilis中的生态功能至关重要.

关键词:

优格伦娜·格拉西利斯 (Euglena gracilis) 是一个有趣的植物.氧酸复合物的复合物.无机酸盐是一种无机酸盐.膜结合式的ecto-phosphatase酶可以通过膜结合.光周期的影响力.

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