相关实验视频

Updated: Jul 20, 2025

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

在胆固醇和酸盐上,β-环氧化改性质的吸附去除特性

Luyao Chen¹, Xu He¹, Yijing Pu¹

¹College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.

|August 4, 2023

概括

一种新的β-cyclodextrin修饰的pectin吸收剂有效地去除胆固醇和胆汁盐. 这种以自然为基础的材料通过增强胃肠道通道健康,对健康食品行业显示出前途.

科学领域:

生物材料科学生物材料科学
食品化学食品化学
吸附科学吸附科学

背景情况:

胃肠道中的胆固醇和胆汁盐可能会影响健康.
pectin是一种天然的多糖,具有一些吸附性质.
修改pectin可以增强其有针对性的分子去除能力.

研究的目的:

通过用β-cyclodextrin修改pectin来合成和描述一种新型吸附剂.
为了评估改性pectin对胆固醇和酸盐的吸附效率.
探索这种吸附剂在健康食品行业的潜在应用.

主要方法:

通过使用谷氨酸的阿尔多尔凝结合成β-环氧化改性pectin.
吸附剂的表面积,热稳定性和细胞兼容性的表征.
吸附实验以确定胆固醇和酸盐的容量,适合兰迈尔和伪二阶模型.

主要成果:

经过修改的pectins表现出增加的特定表面积和热稳定性.
由于疏水性空洞,β-cyclopodextrin的结合显著增强了胆固醇吸附.
与未经修改的pectin或单独的β-cyclodextrin相比,修改过的pectin显示出更好的酸盐吸附.
P_10:1显示,胆固醇的最大吸附能力为44.21 mg/g,酸盐的最大吸附能力为21.38 mg/g.

结论:

经β-cyclopodextrin修饰的pectin是一种有效的胆固醇和胆汁盐的吸附剂.
吸附剂具有适用于胃肠道应用的有利特性.
这种以自然为基础的改性pectin具有在功能性食品和保健补充剂中使用的潜力.

关键词:

吸附剂是一种吸附剂.胆盐:胆盐是一种胆盐.胆固醇胆固醇胆固醇质素 (Pectin) 是一种可怕的物质.胆酸酸是一种酸盐.这是一种β-cyclodextrin.

更多相关视频

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Published on: December 1, 2015

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

相关概念视频

Cellulose and Pectic Polysaccharides

Cellulose and Pectic Polysaccharides

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...

Detergent Purification of Membrane Proteins

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

Size-Exclusion Chromatography

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

Ion Exchange

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Extraction: Advanced Methods

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...