基于石墨烯氧化物的色度/光双模式免疫染色学试验,用于在复杂样品中同时超敏感检测呼吸道病毒和细菌
Xiaodan Cheng1, Xingsheng Yang1, Zhijie Tu1
1Bioinformatics Center of AMMS, Beijing 100850, PR China; Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases, Beijing 100850, PR China.
Journal of hazardous materials
|August 4, 2023
概括
这项研究引入了一种新的双信号生物传感器,用于快速敏感地检测呼吸道病毒和细菌. 基于氧化石墨烯的创新纳米薄膜可实现同时识别,改善护理点诊断.
科学领域:
- 纳米材料科学 科学 纳米材料科学
- 生物传感器技术技术
- 临床诊断 临床诊断 临床诊断 临床诊断
背景情况:
- 目前的临床检测缺乏直接,敏感,同时检测细菌和病毒.
- 需要改善呼吸道病原体的诊断工具.
研究的目的:
- 开发一种具有双信号输出的超敏感免疫染色学试验 (ICA),用于同时检测呼吸道病毒和细菌.
- 为增强生物传感器性能创造一种新的纳米薄膜材料.
主要方法:
- 使用石墨烯氧化物 (GO),金纳米粒子 (AuNPs) 和量子点 (QDs) 制造多层双信号纳米膜.
- 开发一种免疫染色学测定 (ICA) 条带,使用两个测试线进行同时检测.
- 使用色度和光双信号输出进行检测.
主要成果:
- 开发的纳米薄膜提供了增强的表面积,稳定性和分散性.
- 实现了同时检测A型流感病毒和Streptococcus pneumoniae.
- 证明了高灵敏度,检测极限为病毒的891副本/毫升,细菌的17个细胞/毫升.
- 在真实的生物和环境样本中成功应用.
结论:
- 拟议的双模式ICA提供了一种灵活而准确的方法来检测呼吸道病原体.
- 双信号方法扩大了ICA的适用于快速选和敏感量化的应用.
- 生物传感器显示出在临床诊断中实地应用的巨大潜力.
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