通过高速反流色谱与不同的旋转模式,直接从生原料提取物中制备大规模低极性生的高效策略
Jisheng Li1, Qiqi Wang2, Yao Wang3
1Key Laboratory of Comprehensive and Highly Efficient Utilization of Salt Lake Resources, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences, Xining, P. R. China.
Journal of separation science
|August 4, 2023
概括
这项研究开发了一种高效的方法,使用高速逆流色谱从生中分离出低极性生醇. 这种技术成功地在一次运行中净化了五种主要的生,提供了可扩展的制备策略.
科学领域:
- 自然产品化学 自然产品化学
- 染色学分离科学 染色学分离科学
背景情况:
- 生醇是生中发现的生物活性化合物,具有各种健康益处.
- 需要有效和可扩展的方法来从原始提取物中分离特定的生.
研究的目的:
- 开发一种高效的大规模制备策略,直接从生原料提取物中制备低极性生醇.
- 优化提取和分离条件,以获得最大的产量和纯度.
主要方法:
- 超声波辅助提取 (UAE) 通过响应表面方法 (RSM) 优化.
- 高速反流色谱 (HSCCC) 使用特定的溶剂系统 (n-hexane/乙烯/甲醇/水, 6:4:6:4 v/v/v/v) 和不同的旋转模式.
- 使用1H-NMR光谱的结构识别.
主要成果:
- 优化的阿联条件有效丰富了主要的低极性生醇.
- 在一次运行中,HSCCC成功地分离了五种主要的生醇 ([6]-生醇, [8]-生醇, [6]-shogaol, [6]-dehydrogingerdione, [10]-生醇).
- 产量包括150毫克的 [6] - gingerol,50毫克的 [8] - gingerol,20毫克的 [6] - shogaol,43毫克的 [6] - dehydrogingerdione,和40毫克的 [10] - gingerol从1.2g的原始提取物在400分钟内.
结论:
- 该研究提出了一个高效且可扩展的HSCCC战略,用于从生原料提取物直接制备低极性生醇.
- 优化的方法允许同时分离具有识别结构的多个有价值的生醇.
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