来自Lysimachia christinae Hance Hance的酸性异多糖糖的结构特征和抗补活性
Zhou Hong1,2, Li-Shuang Zhou2, Zhi-Zhi Zhao2
1State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China.
Planta medica
|August 4, 2023
概括
来自Lysimachia christinae的一种新型酸性异多聚糖体表现出强大的抗补和抗氧化活性. 增加的葡萄糖酸含量增强了这些生物活性,这表明它在植物中的重要性.
科学领域:
- 植物化学 植物化学
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 莱西马希亚克里斯蒂娜 (Lysimachia christinae Hance) 是一种以"清除热量"的特性而闻名的传统中医药.
- 多聚糖是复杂的碳水化合物,具有多样化的生物活动.
- 了解植物性多糖的结构-活性关系对于药物发现至关重要.
研究的目的:
- 从Lysimachia christinae中分离和鉴定一种新型的酸性异多聚糖化物 (LCP-90-1).
- 研究LCP-90-1及其衍生物的抗补和抗氧化活性.
- 阐明葡萄糖酸含量在观察到的生物活性中的作用.
主要方法:
- 隔离和净化LCP-90-1.1的异多聚糖.
- 结构分析包括单糖组成和链接分析.
- 在体外测定抗补剂活性 (CH50) 和抗氧化活性.
- 酶和酸性水解产生降解产品.
主要成果:
- LCP-90-1是一种酸性异多聚糖化物 (Mw 20.65 kDa),由Man,Rha,GlcA,Glc,Gal和Ara组成.
- LCP-90-1在体外显示出强大的抗补充活性 (CH50: 135.01 ± 0.68 μg/mL).
- 降解产物LCP-90-1-A,具有增加的GlcA含量,显示显著增强抗补剂 (CH50:28.26±0.39μg/mL) 和抗氧化活性.
- 活动取决于GlcA的完整性,对还原或水解敏感.
结论:
- 葡萄糖酸含量在LCP-90-1的抗补和抗氧化活性中起着关键作用.
- LCP-90-1及其富含GlcA的衍生物是来自Lysimachia christinae的潜在治疗剂.
- 来自L. christinae的高GlcA含量的多糖体需要进一步研究它们的药用价值.
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