通过比较耐热性能和全转录组RNA测序来调节海 (Strongylocentrotus intermedius) 的热应激反应的分子机制

相关实验视频

Updated: Jul 20, 2025

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans

Published on: July 3, 2020

Lingshu Han¹, Pengfei Hao², Wenpei Wang²

¹Key Laboratory of Northern Aquatic Germplasm Resources and Genetic Breeding in Liaoning Province, Dalian 116023, China; Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, Liaoning, 116023, China; School of Marine Sciences, Ningbo University, Ningbo 315211, China.

The Science of the total environment

|August 4, 2023

概括

海通过基因表达的变化表现出耐热性. 这项研究揭示了分子机制,包括ceRNA网络,参与水产养殖应用的热应激反应.

科学领域:

海洋生物学海洋生物学
基因组学就是基因组学.
适应气候变化适应气候变化

背景情况:

海种群,特别是*Strongylocentrotus intermedius*,由于中国北部的极端温度而面临高死亡率.
了解耐热机制对于水产养殖和保护工作至关重要.

研究的目的:

在整个转录组层面研究 *Strongylocentrotus intermedius* 耐热性背后的分子机制.
识别涉及热应激反应的差异表达基因和非编码RNA.
构建一个具有竞争力的内源RNA (ceRNA) 调节网络,以阐明调节途径.

主要方法:

全转录组RNA测序在*S. intermedius*的耐热和对照组上进行.
在热应力下对信使RNAs (mRNAs),microRNAs (miRNAs) 和长非编码RNAs (lncRNAs) 的差异表达分析.
基于差异表达分子之间的预测相互作用构建一个ceRNA网络.

主要成果:

在应对热应激时识别出差异表达的mRNA,miRNA和lncRNA.
构建了一个ceRNA调节网络,揭示了分子相互作用.
功能分析表明,ceRNAs参与基底代谢,离子运输,内质网膜应激和亡.

结论:

这项研究提供了*S. intermedius*在热应力下进行的全面全转录组分析.
拟议的ceRNA网络提供了对耐热性分子调节机制的见解.
这些发现为进一步研究海的环境反应和适应中的lncRNA和miRNA功能奠定了基础.

关键词:

热应激是一种热应激.斯特朗基洛中心的中间体 (intermedius)整个转录组RNA-seqq.ceRNARNA是什么意思在ncRNA中,我们可以这是一个小RNARNA.

更多相关视频

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris

Published on: January 3, 2025

Measurements of Physiological Stress Responses in C. Elegans

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

相关概念视频

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Other Stress Responses in Bacteria

Other Stress Responses in Bacteria

Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...