在Pseudomonas amygdali pathovar aesculi形状冠状体等离子体中的遗传重组
Tue Kjærgaard Nielsen1, Caroline S Winther-Have2, Iben Margrete Thomsen3
1Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
概括
植物致病性 伪omonas物种通过等离子体调动病毒性基因. 这项研究揭示了植物毒素冠状素基因集群最近如何转移到等离子体中,帮助细菌适应和感染.
科学领域:
- 微生物学 微生物学
- 植物病理学 植物病理学
- 基因组学就是基因组学.
背景情况:
- 植物致病性 伪omonas物种利用毒素和毒性因子来感染宿主.
- 致病性基因通常在移动遗传元素上,通过横向基因转移获得进化优势.
- 调动这些基因的遗传机制尚未完全理解.
研究的目的:
- 分析四种Pseudomonas amygdali pv.的完整基因组序列. 一个esculi (Pae) 菌株. 一个esculi (Pae) 菌株.
- 调查Pae.e.病毒性因子调动的遗传基础.
- 为了理解 Pseudomonas 病原性基因的进化动态.
主要方法:
- 四个Pae菌株的全基因组测序.
- 对基因组数据的生物信息分析,专注于等离子体和移动遗传元素.
- 比较基因组学以确定基因转移事件.
主要成果:
- 四个Pae基因组包含六个等离子体组,它们都编码了致病性因素.
- 效应基因经常与插入序列元素相关,表明动员.
- 冠状腺生物合成基因群最近从染色体转移到可调动的等离子体中,然后与结合性等离子体集成.
结论:
- 形桃体病毒 pv. aesculi利用等离子体传播的毒性因子来感染.
- 插入序列在将致病性基因调动到等离子体上起着关键作用.
- 最近的水平基因转移,以冠状腺基因集群为例,促进了细菌的适应和进化.
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