一种小型化样本制备方法,用于在全血中使用ICP-QQQQ的体积吸收微型采样进行常规的元素测定
Lucas Schmidt1, Kayla Peterson1, Thieli Schaefer Nunes1
1Department of Environmental Medicine and Public Health, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
Analytical and bioanalytical chemistry
|August 4, 2023
概括
这项研究引入了一种新方法,用于分析采用体积吸收微量采样 (VAMS) 收集的血液样本中的微量元素. 该技术提供了准确和精确的元素定量,推动了临床和生物分析研究.
科学领域:
- 分析化学 分析化学
- 生物医学科学 生物医学科学
- 环境科学 环境科学
背景情况:
- 体积吸收微型采样 (VAMS) 是采集血液的安全简单方法.
- 精确确定血液中必需和非必需的微量元素对于临床和生物分析应用至关重要.
- 现有的方法在样品制备和VAMS样品中微量元素的分析灵敏度方面可能存在局限性.
研究的目的:
- 开发和验证一种用于确定VAMS收集的血液样本中的15种基本和非基本微量元素的新方法.
- 建立使用微波辅助消化和ICP-QQQ的强大而敏感的分析程序.
- 与传统技术相比,评估拟议方法的准确性,精度和可靠性.
主要方法:
- 微波辅助消化使用稀释酸混合物 (4.5 mol L-1 HNO3 + 100 μL H2O2 30%) 对VAMS血液样本进行消化.
- 使用感应合等离子体三重四极质谱法 (ICP-QQQ) 进行元素测定.
- 通过参考材料分析,回收试验和与液体采样方法的比较进行验证.
主要成果:
- 该方法实现了15种微量元素 (As,Be,Cd,Cs,Cu,Fe,Mg,P,Pb,S,Sb,Se,Tl,V,U) 的高精度和准确性.
- 消化效率>95%,稀释系数低 (30倍),适合微分析.
- 结果与参考值一致,并根据霍维茨方程证明了良好的精度.
- 不需要矩阵匹配的校准曲线,简化了分析过程.
结论:
- 开发的基于VAMS的方法为血液中微量元素分析提供了灵敏,准确和精确的方法.
- 该方法在生物安全性,实用性和稳定性方面为生物医学和临床应用提供了优势.
- 这种创新技术推进了微量元素分析,支持其在各种研究和诊断环境中使用.
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