辅助因子Ycf48在光系统II生物发生过程中占据了氧气进化的团的位置
Ziyu Zhao1, Irene Vercellino2,3, Jana Knoppová4
1Sir Ernst Chain Building-Wolfson Laboratories, Department of Life Sciences, Imperial College London, S. Kensington Campus, London, SW7 2AZ, UK.
Nature communications
|August 4, 2023
概括
在组装过程中,Ycf48蛋白通过与D1多结合来保护光系统二 (PSII) 复合体. 这可以防止过早的和结合,确保强大的光合作用.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 光合作用研究研究 光合作用研究
背景情况:
- 氧化光合作用依赖于辅助因素,以实现高效的第二光系统 (PSII) 组装和修复.
- 已知保存的Ycf48蛋白与D1反应中心聚结合,有助于PSII组合,但其精确的结合点和功能仍然难以捉摸.
研究的目的:
- 阐明Ycf48组合因子在光系统二 (PSII) 复合体形成的早期阶段的结合部位和结构作用.
- 了解Ycf48如何促进D1/D2反应中心复合体的组装,并保护催化部位.
主要方法:
- 使用冷电子显微镜 (cryo-EM) 来确定与Ycf48.8结合的蓝藻PSII D1/D2反应中心组装复合物的高分辨率结构.
- 结构分析的重点是Ycf48,D1和D2多之间的相互作用.
主要成果:
- 解决了Ycf48的7叶片β螺旋结构.
- 发现Ycf48与将Mn4CaO5集群结合的D1残留物结合,从而在固体上阻碍过早的Mn2+和Ca2+离子结合.
- 确定了Ycf48和D2之间的相互作用,澄清了它在D1/D2复杂组装中的作用.
结论:
- 在早期PSII组装过程中,Ycf48充当了关键的陪伴者,占据了D1上新生的Mn4CaO5结合点,防止损伤和过早的金属结合.
- 结构洞察力揭示了D1/D2复合体中的动态变化,这些变化建立了水氧化的催化部位.
- 这项研究提供了对光合作用复杂生物发生过程中的辅助因子功能的机械学理解.
更多相关视频
10:20Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
Published on: August 9, 2019
12.7K
11:28Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids
Published on: August 28, 2018
7.8K
相关概念视频
Photosystem I
63.0K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
63.0K
Photosystem II
71.1K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
71.1K
Photosystems
4.9K
Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
4.9K
The Antenna Complex
6.0K
Plants and other photosynthetic organisms comprise pigments capable of absorption of direct sunlight. These pigments are present in the reaction center - the main site of photochemical reactions as well as in the antenna complex. Under average light conditions, the rate at which reaction center pigments absorb light is far below the electron transport chain's capacity. As a result, the reaction center alone cannot provide enough energy to drive photosynthesis. The photosynthetic efficiency...
6.0K
The Photochemical Reaction Center
4.2K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.2K
The Z-Scheme of Electron Transport in Photosynthesis
10.2K
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
10.2K
