一个转化调节器MHZ9调节中的乙烯信号
Yi-Hua Huang1, Jia-Qi Han1,2, Biao Ma3
1State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Innovative Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
Nature communications
|August 4, 2023
概括
研究人员发现了MHZ9,一种控制大米基因翻译的蛋白质. 这一发现增强了我们对乙烯信号的理解,有助于应激适应和作物改善.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 乙烯对的生长,发育和应激反应至关重要.
- 在大米乙烯信号中,转化控制的精确机制尚未完全理解.
研究的目的:
- 确定米在翻译水平上乙烯信号的新型调节剂.
- 为了阐明识别的调节器的功能和机制,MHZ9.9.
主要方法:
- 对乙烯反应突变物 (mhz9) 的分析.
- 甘氨酸-氨酸-氨酸 (GYF) 域蛋白MHZ9.9的识别和表征.
- 定位研究 (RNA处理器官).
- 蛋白质与蛋白质相互作用试验 (OsEIN2).
- RNA结合试验 (OsEBF1/2mRNAs) 进行.
- 高通量测序 (RNA-IP 序列,CLIP 序列,Ribo 序列).
主要成果:
- MHZ9在翻译层面积极调节乙烯信号.
- MHZ9与OsEIN2相互作用,并与OsEBF1/2mRNAs结合,抑制它们的翻译.
- 这种抑制允许OsEIL1的积累,激活下游的乙烯信号.
- MHZ9与数百种RNA相关,对于约90%的乙烯反应基因的翻译调节至关重要.
结论:
- MHZ9是大米乙烯信号传输中的关键翻译调节器.
- MHZ9调解了许多基因对乙烯的反应的翻译控制.
- 这种MHZ9的调节作用对于米应激适应和潜在的特征改善至关重要.
相关概念视频
Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
Riboswitches
8.2K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.2K
Translational Regulation
45
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
45


