一个多omics分析识别了与 (Bos taurus) 的生育相关的分子特征
Mackenzie A Marrella1, Fernando H Biase2
1School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Scientific reports
|August 4, 2023
概括
牛不孕会影响牛的生产. 多omics分析确定了与生育相关的遗传标记和分子概况,提供了超越品种特定遗传学的见解.
科学领域:
- 动物科学动物科学
- 遗传学 是一个遗传学.
- 生物技术是生物技术.
背景情况:
- 母不孕症是畜牧业的一个重大经济挑战.
- 了解不孕症的遗传和生理基础对于可持续的牛畜生产至关重要.
- 雌牛作为一个有价值的生物医学模型来研究由于固有的原因导致的不孕症.
研究的目的:
- 确定与母牛生育相关的遗传和分子因素.
- 通过采用多学科方法,研究母牛不孕症的根本原因.
- 开发一种用于预测母牛生育能力的分子概况.
主要方法:
- 用高密度单核酸多态 (SNP) 芯片收集基因组数据.
- 使用PLINK与费舍尔精确测试进行了关联分析.
- 使用统计测试和通用混合模型分析了定量转录组和蛋白质组数据.
主要成果:
- 两个SNP (rs110918927和rs109366560) 与母牛的生育能力有显著的关联.
- 在肥沃和不肥沃的公牛之间观察到APMAP和DNAI7基因的不同转录丰度.
- 蛋白质FTO在肥沃的母牛中比较丰富,整合性分析在22只母牛中正确分类了21只母牛的生育能力.
结论:
- 多omics分析证实了母牛生育能力的复杂性质.
- 识别的分子特征 (SNP,转录,蛋白质) 可以区分肥沃和不肥沃的小母牛.
- 这些发现突出了与生育相关的分子差异,这些差异独立于特定品种的遗传背景.
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