XCP1将与致病性相关的蛋白1切割到CAPE9中,以获得Arabidopsis中的系统性免疫力
Ying-Lan Chen1,2, Fan-Wei Lin1, Kai-Tan Cheng1
1Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan.
Nature communications
|August 4, 2023
概括
植物免疫力依赖于蛋白质酶,如西囊酶1 (XCP1) 来激活细胞因子. XCP1处理与病原性相关的蛋白1 (PR1) 释放CAPE9,这对全身免疫至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 通过蛋白质分解激活细胞因子对于动物的免疫力至关重要,涉及caspases.
- 植物免疫力也需要蛋白质溶解性细胞因子的产生,但对调节免疫力的植物细胞因子的证据有限.
研究的目的:
- 调查依赖氨酸的亚斯巴酸特异蛋白酶在植物免疫中的作用.
- 确定负责处理病变相关蛋白1 (PR1) 的特定蛋白酶及其在植物免疫反应中的作用.
主要方法:
- 在Arabidopsis中分析PR1的C端加工.
- 酶活性测定西莱姆氨酸化酶1 (XCP1).
- 评估XCP1对系统性免疫诱导的要求.
主要成果:
- 在PR1中"CNYD"基因的蛋白质分解处理产生免疫调节性细胞因子CAPE9.9.
- 酸增强了针对CNYD动机和CAPE9释放的蛋白酶的活性.
- 鉴定出西半氨基酶1 (XCP1) 是负责CAPE9生成的类蛋白酶.
- XCP1的活性是调节的,与人类的caspases相当.
- XCP1对于诱导由病原体相关的分子模式触发的全身免疫是必不可少的.
结论:
- 糖囊素酶1 (XCP1) 是植物免疫中的一个关键蛋白酶.
- XCP1从PR1中产生细胞因子CAPE9,从而激活全身免疫力.
- 这项研究揭示了植物免疫调节的新机制,涉及酶类蛋白酶和细胞因子生产.
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