对Mycobacterium结核病原生蛋白的抗体可以检测肺结核病患者的肺结核病
Desak Nyoman Surya Suameitria Dewi1,2, Ni Made Mertaniasih3,4, Soedarsono5
1Department of Bacteriology, School of Medicine, Niigata University, Asahimachi-Dori 1-757, Chuo-ku, Niigata, 951-8510, Japan. desak.nyoman@ciputra.ac.id.
开发精确的结核病 (TB) 治疗点测试 (POCT) 需要使用本地Mycobacterium结核病蛋白质. 原生Rv1860和Ag85B蛋白显示出结核病检测的诊断潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 医学诊断 医学诊断 医学诊断
背景情况:
- 准确的护理点检测 (POCT) 对于结核病 (TB) 管理至关重要.
- 目前基于抗体的结核病诊断方法在特异性和敏感性方面存在局限性.
研究的目的:
- 确定适合的Mycobacterium结核病 (M.tb) 抗原候选人用于基于抗体的结核病诊断.
- 评估肺结核 (PTB) 患者对M.tb蛋白的IgG抗体反应.
主要方法:
- 在PTB患者中对主要M.tb分泌蛋白质 (Rv1860,Ag85C,PstS1,Rv2878c,Ag85B,Rv1926c) 的评估IgG反应.
- 使用ROC分析对原生与重组抗原的诊断性能进行比较.
- 测试了59名PTB患者和102名健康对照 (HC).
主要成果:
- 在PTB患者中,IgG水平与本地Rv1860,PstS1和Ag85B相比显著升高.
- 重组PstS1和Ag85B未能将PTB患者与HC患者区分开来.
- 原生Rv1860和Ag85B显示可接受的诊断准确性 (AUC分别为0.821和0.812).
结论:
- M.tb抗原的原生结构对于开发有效的基于抗体的结核病POCT至关重要.
- 在原生形式使用时,Rv1860和Ag85B显示为结核病POCT的诊断目标.
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