贝西迪奥米塞特非还原性多基基化合成酶的功能独立于SAT域
Nikolai A Löhr1,2, Malik Rakhmanov1,2, Jacob M Wurlitzer1,2
1Institute of Pharmacy, Department Pharmaceutical Microbiology, Friedrich Schiller University Jena, Winzerlaer Strasse 2, 07745, Jena, Germany.
起始单元:乙转移酶 (SAT) 域对于许多真菌非还原聚基酸合成酶 (NR-PKSs) 不是必不可少的,特别是在Basidiomycota. 这一发现影响了天然产品发现和酶工程策略.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 非还原性多基合成酶 (NR-PKSs) 是真菌天然产品生物合成中的关键酶,是Asco和Basidiomycota中的多样化化合物.
- 传统上,NR-PKS通过一个N端起始单元:乙转移酶 (SAT) 域启动聚乙化物合成,将乙基转移到乙载体蛋白 (ACP).
研究的目的:
- 研究不同真菌系的NR-PKS中SAT域的功能必要性.
- 从一个新发现的第十二个基团中对新型NR-PKS进行鉴定.
主要方法:
- 对NR-PKS序列的遗传学分析.
- 在体内和体外对野生类型和无SAT域的NR-PKS变体的生物化学表征.
- 对ascomycete和basidiomyceteNR-PKSs进行比较分析.
主要成果:
- 确定了NR-PKS的第十二个基团,其中来自Cortinarius rufoolivaceus的特征酶缺乏功能性SAT域,但仍然活跃.
- 来自Basidiomycota (clade VIII) 的无SAT域的NR-PKS保留了完全的酶活性.
- 一个没有SAT域的ascomyceteNR-PKS (ACAS) 是不活跃的,这表明SAT域的血统特定的角色.
结论:
- 对于许多基菌体NR-PKS的功能来说,SAT域是不可或缺的,与ascomycetes不同.
- 这种理解可以指导为合成生物学设计更高效的NR-PKS系统.
- 应考虑对无SAT域的NR-PKS基因进行基因识别,以了解真菌天然产品的潜力,避免将其误归为伪基因.
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