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Updated: Jul 20, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

总RNA测序揭示了基因表达和微生物变化,这些变化与口腔前恶性病变和癌症共同存在

Mohammed Muzamil Khan^1,2, Jennifer Frustino³, Alessandro Villa^4,5

¹Section of Computational Biomedicine, Boston University Chobanian & Avedisian School of Medicine, 75 E. Newton St. E609, Boston, MA, 02118, USA.

|August 4, 2023

概括

这项研究揭示了口腔前恶性病变 (PMLs) 的分子变化,可能导致口腔癌 (OC). 了解这些变化,包括细胞可塑性和微生物组的联系,可以使口腔状细胞癌 (OSCC) 的早期发现和干预成为可能.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
微生物组研究微生物组研究

背景情况:

口腔癌 (OC) 是一种致命和毁容的恶性瘤,通常是由前恶性病变 (PML) 引起的.
目前口腔状细胞癌 (OSCC) 的早期检测和治疗方法不足,导致~65%的5年生存率.
在发展为OSCC之前,PMLs通过包括上皮质发育不良在内的组织病理阶段进行进展.

研究的目的:

研究导致口腔前恶性病变 (PMLs) 进展为口腔状细胞癌 (OSCC) 的分子机制.
为了确定口腔癌的早期诊断和疾病拦截的潜在生物标志物.

主要方法:

66个人类PML的全转录组概况 (带有发育不良的白血病和非反应性皮质增生症),健康对照和OSCC.
对宿主转录组和口腔微生物组数据的综合分析.

主要成果:

PMLs表现出与细胞可塑性相关的丰富基因特征,包括部分上皮层-介质细胞转换 (p-EMT) 现型和免疫反应.
在特定的口腔微生物的丰富性和PML通路的活性之间发现了显著的关联.
口腔微生物组似乎有助于PMLs演变为OSCC.

结论:

导致PML发展为OSCC的分子过程涉及细胞可塑性和宿主微生物群相互作用.
这些发现提供了对口腔癌潜在的早期诊断标志物和治疗点的见解.
了解这些分子通路可能有助于更早地拦截疾病并改善患者的治疗结果.

关键词:

早期检测早期检测口腔癌是一种口腔癌.口腔的微生物群.恶性病变前的病变

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