从重复流感疫苗接种中描述BCR谱的动态
Guoqin Mai1,2, Chi Zhang1,2, Chunhong Lan3,4,5
1School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, People's Republic of China.
Emerging microbes & infections
|August 5, 2023
概括
重复接种流感疫苗会引起不同的B细胞受体 (BCR) 谱系动态. 第一次疫苗接种显示了更广泛的变化,而第二次疫苗接种则突出了像IGHV1-69这样的特定基因段,用于潜在的广泛中和抗体.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
背景情况:
- 季节性流感对全球健康造成重大负担.
- 优化流感疫苗接种策略需要了解对重复接种疫苗的免疫反应.
研究的目的:
- 为了研究连续接种流感疫苗后B细胞受体 (BCR) 谱的动态.
- 确定关键的B细胞基因段和异型,参与免疫反应对初级和二级疫苗接种.
主要方法:
- 在两个流感季节的16名接种疫苗的志愿者中追踪BCR谱系动态 (多样性,长度,基因使用,体质突变,同型).
- 分析V基因段的使用 (IGHV4-39,IGHV3-9,IGHV3-7,IGHV1-69) 和同型贡献 (IgM,IgG3,IgG1,IgG2).
- 识别公共BCR集群和表征互补性确定区域3 (CDR3) 动机.
主要成果:
- 与第二次接种相比,在第一次疫苗接种的急性阶段,BCR谱的变化更为明显.
- 在第一次疫苗接种的急性阶段,特定的V基因段 (IGHV3-7主导) 被放大,与IgM和IgG3相关.
- 第二次接种疫苗的变化主要由IGHV1-69主导,与IgG1和IgG2相关,这表明具有广泛中和抗体的潜力.
- 确定了41个涉及IGHV3-7和IGHV1-69的公共BCR集群.
结论:
- 对重复接种流感疫苗的免疫反应表现出不同的动态,第二次接种疫苗可能会为更广泛的保护做出准备.
- 特定的BCR基因段和同型在对流感疫苗的初级和二级免疫反应中起着不同的作用.
- 研究结果为设计改进的全民流感疫苗和疫苗接种策略提供了洞察力.
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