转录因子ERF108与AUXIN RESPONSE FACTORs相互作用,以调解棉纤维二次细胞壁生物合成
Yao Wang1, Yang Li1, Shao-Ping He1
1Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079,China.
The Plant cell
|August 5, 2023
概括
由 GhERF108 和 GhARF7s 介导的乙烯和奥克辛信号交叉,激活 GhMYBL1,促进棉纤维二次细胞壁生物合成.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 植物激素对植物发育至关重要.
- 棉纤维二次细胞壁 (SCW) 形成中的植物激素调节机制尚未完全理解.
研究的目的:
- 研究棉纤维SCW生物合成中的乙烯和奥辛信号之间的分子相互作用.
- 阐明 GhERF108, GhARF7-1, GhARF7-2 和 GhMYBL1 在这个过程中的作用.
主要方法:
- 在实验室中培养棉花卵子.
- 棉花中的RNA干扰 (RNAi) 和病毒诱导的基因沉默 (VIGS).
- 蛋白质与蛋白质相互作用的测试.
- 促进剂结合测定试验
主要成果:
- 乙烯和奥素促进棉纤维中的SCW沉积.
- GhERF108与GhARF7-1/GhARF7-2相互作用,以激活GhMYBL1的表达.
- GhMYBL1直接激活了纤维素生物合成基因 (GhCesA4-1, GhCesA4-2, GhCesA8-1).
- 抑制GhERF108,GhARF7-1/GhARF7-2或GhMYBL1会影响SCW的形成.
结论:
- 一个涉及GhERF108,GhARF7-1/GhARF7-2和GhMYBL1的新型乙烯-auxin信号交叉通路调节棉纤维SCW生物合成.
- 这一途径激活了纤维素生物合成,增强了SCW沉积.
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