相关实验视频

Updated: Jul 20, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

在肝细胞癌中对CPSF4相关的替代拼接基因进行全面分析

Anwaier Yuemaierabola^1,2, Jun Guo^1,2, Lili Sun^1,2

¹Department of Cancer Research Institute, Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi, 830011, China.

Journal of cancer research and clinical oncology

|August 5, 2023

概括

异常替代拼接 (AS) 与肝细胞癌 (HCC) 有关. 这项研究确定了七个与CPSF4相关的AS基因,这些基因可以预测HCC的预后和免疫透,提供潜在的诊断和治疗生物标志物.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
生物信息学是一种生物信息学.

背景情况:

替代拼接 (AS) 对于基因表达控制至关重要,其失调有助于肝细胞癌 (HCC) 的发展.
裂变和多化特异因子4 (CPSF4) 是一个关键的AS调节器,但其在HCC免疫微环境透中的作用尚不清楚.

研究的目的:

研究CPSF4相关的替代拼接 (AS) 分子与肝细胞癌 (HCC) 瘤微环境 (TME) 中的免疫细胞透之间的关联.
根据与CPSF4.4相关的AS基因开发HCC的预后模型.

主要方法:

分析了来自TCGA-LIHC的RNA测序和临床数据,以确定差异表达的AS基因.
预后性AS基因使用单变Cox,卡普兰-梅尔 (KM) 分析和斯皮尔曼相关性来确定.
使用Cox和逐步回归构建了一个预后预测模型,并评估了它与TME细胞透的相关性.

主要成果:

一个七基因的AS模型 (STMN1,CLSPN,MDK,RNFT2,PRR11,RNF157,GHR) 被开发来预测HCC的预后,高风险组显示明显更差的结果 (p < 0.0001).
临床诺米图表表现出1,2年和3年HCC患者存活率的良好预测性能 (AUC:0.76,0.70,0.69).
预后特征与免疫透和免疫检查点基因表达有显著的关联.

结论:

这项研究阐明了CPSF4和七个AS基因在HCC发育和进展中的作用.
鉴定的AS基因和预后模型可以作为HCC预后,诊断和潜在治疗的宝贵生物标志物.

关键词:

替代拼接 (AS) 替代拼接在CPSF4中使用.肝细胞癌 (HCC) 是一种肝细胞癌.免疫系统的透.预测预后预测预测

更多相关视频

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

相关概念视频

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...