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来自莫斯克的多可以通过抑制NF-κ B-ROS/NLRP3通路来抑制脂多糖诱导的炎症

Jing Yi¹, Li Li², Zhu-Jun Yin^2,3

¹Department of Pharmacology, Southwest Medical University, Luzhou, Sichuan Province, 646000, China.

Chinese journal of integrative medicine

|August 5, 2023

概括

莫斯克的多 (PPM) 通过抑制NF-κB和NLRP3.3等关键通路,有效降低炎症. 这项研究表明,PPM是潜在的炎症性疾病治疗剂.

科学领域:

药理学药理学是指药理学的学科.
免疫学免疫学免疫学
生物化学生物化学

背景情况:

炎症是一种复杂的生物反应,与许多疾病有关.
脂多糖 (LPS) 是一种强大的炎症诱导剂.
需要针对炎症途径的新型治疗剂.

研究的目的:

为了研究来自莫斯科的多 (PPM) 的抗炎作用.
阐明PPM在LPS引起的炎症中的作用的潜在机制.
在体外和体外模型中评估PPM的疗效.

主要方法:

使用染色学和电泳法提取和表征多.
使用LPS诱导的THP-1巨细胞进行体外研究,以评估细胞活力,细胞因子产生,ROS水平和通路激活 (NF-κB,TXNIP/NLRP3).
使用LPS诱导的BALB/c小鼠进行体内研究,以测量血清和肺细胞因子,并分析肺组织学.

主要成果:

在LPS刺激的巨细胞中,PPM (10-26 kD) 显著降低了促炎性细胞因子 (IL-1β,IL-18,TNF-α,IL-6) 和ROS.
通过降低关键蛋白质和mRNA表达,PPM抑制了NF-κB和TXNIP/NLRP3炎症酶通路.
在体内,PPM缓解了肺炎,并降低了LPS治疗小鼠的炎症性细胞因子水平.

结论:

通过抑制NF-κB-ROS/NLRP3信号级联,PPM显示出显著的抗炎性质.
在细胞和动物模型中,PPM有效地减轻LPS诱导的炎症.
PPM代表了开发新型抗炎疗法的有希望的候选人.

关键词:

中国医学中医药是什么?莫斯库斯 (Moschus) 是一种植物.在NLRP3炎症酶体中,NLRP3炎症这是一种炎症炎症炎症炎症.核因子 κB 核因子 κB 这是一种多类聚.铁素相互作用蛋白质.

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