孕妇和哺乳期阿瓦西母羊的OXT基因多态和生殖激素之间的关系
Neam M Khazaal1, Hasan F Alghetaa1, Mohammed Baqur S Al-Shuhaib2
1Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Molecular biology reports
|August 5, 2023
概括
在阿瓦西羊群中,在催产素基因 (OXT) 中发现了一种新的单核酸多态 (SNP). 这种OXT基因变异会对生殖激素水平产生负面影响,为羊养殖计划提供了洞察力.
科学领域:
- 动物遗传学动物遗传学
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
背景情况:
- 哺乳动物的繁殖涉及复杂的遗传相互作用.
- 催产素基因 (OXT) 是生殖特征的关键候选者.
- 关于在绵羊和山羊中OXT多态性的先前研究存在,但在阿瓦西羊中没有.
研究的目的:
- 为了研究阿瓦西羊的OXT基因多态性.
- 确定OXT多态性和子大小对孕妇和哺乳期阿瓦西羊的生殖激素的影响.
主要方法:
- 评估了232只阿瓦西羊 (3-4岁;123只,109只双生产).
- 使用ELISA测量了血清生殖激素 (雌激素,孕,FSH/LH).
- 提取的DNA用于OXT基因测序 (外显子2) 和基因定型.
主要成果:
- 鉴定了三种OXT基因型 (CC,CA,AA) 和一个新突变 (188 C>A SNP) 在异构2中.
- 188 C>A SNP与表型特征有显著的关联.
- 与CA/AA基因型相比,具有CC基因型的双胞胎怀孕母羊的雌激素,孕和FSH/LH水平较高.
结论:
- 阿瓦西羊OXT基因中的188C>A SNP对生殖激素水平产生负面影响.
- 这些发现为阿瓦西羊的选择性繁殖提供了宝贵的见解.
- 这项研究有助于了解OXT基因在绵羊繁殖中的作用.
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