相关实验视频

Updated: Jul 20, 2026

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

在SARS-CoV-2中,利用来自不同蝙蝠物种的ACE2可以使病毒进入体外并进行复制

Kelsey Briggs¹, Ryan Sweeney¹, David S Blehert²

¹Exotic and Emerging Avian Disease Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Center, Agricultural Research Service, USDA, 934 College Station Road, Athens, GA, 30605, USA.

|August 5, 2023

概括

有七种蝙蝠物种.

科学领域:

病毒学病毒学
动物传播疾病动物传播疾病
进行比较基因组学.

背景情况:

严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 被怀疑是动物源的,蝙蝠作为潜在的自然储库.
了解蝙蝠物种对SARS-CoV-2的易感性对于流行病准备和One Health倡议至关重要.

研究的目的:

在实验室中调查七种蝙蝠物种对SARS-CoV-2感染的 ангиотензин转化酶2 (ACE2) 受体的易感性.
建立一个实用的体外方法来评估动物物种对SARS-CoV-2及其变体的敏感性.

主要方法:

来自七种蝙蝠物种的ACE2受体 (小棕色,大圆叶,皮尔森马,大马,巴西自由尾,埃及,和中国粗马) 在DF1细胞中被单独表达.
表达蝙蝠或人类ACE2的DF1细胞被SARS-CoV-2 (武汉血统和变种) 挑战,以评估病毒的附着和复制.

主要成果:

所有七个测试的蝙蝠ACE2受体都使得DF1细胞对SARS-CoV-2感染具有宽容性.
蝙蝠物种和SARS-CoV-2变种之间病毒复制水平有所不同.
与测试的变种相比,武汉血统显示出更高的复制量.
人类ACE2支持的病毒标位明显高于任何测试的蝙蝠ACE2.

结论:

研究的蝙蝠物种的ACE2受体可以支持SARS-CoV-2的复制,表明潜在的易感性.
这种体外系统为评估SARS-CoV-2宿主范围和监测新出现的病毒威胁提供了宝贵的工具.

关键词:

在ACE2中,ACE2是ACE2.这就是为什么BATBATBAT.感染感染感染模型模型模型模型模型复制复制复制复制复制复制复制这就是SARS-CoV-2病毒.

更多相关视频

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Published on: November 5, 2021

Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro

Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro

Published on: June 6, 2025

相关概念视频

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

Coronavirus

Coronavirus

Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...