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对Brachionus asplanchnoidis (Rotifera) 紫外线B辐射的生理反应和分子机制

Dongqi Kan¹, Yu Zhang¹, Jiancheng Zeng¹

¹School of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, People's Republic of China.

Ecotoxicology and environmental safety

|August 5, 2023

概括

紫外线B (UV-B) 辐射会损害虫的生长和繁殖,尽管它们具有耐受性. 生物体表现出氧化损伤和DNA修复激活作为UV-B暴露的反应.

科学领域:

环境科学环境科学
分子生物学分子生物学
生态毒理学生态毒理学

背景情况:

紫外线B (UV-B) 辐射对水生生物造成重大环境压力.
了解生物对UV-B的反应对于评估生态影响至关重要.

研究的目的:

为了研究UV-B辐射对单体 rotifer Brachionus asplanchnoidis 的生理和分子效应.
阐明UV-B反应的机制,包括抗氧化系统,DNA损伤和修复途径.

主要方法:

暴露于不同剂量的UV-B辐射.
评估生存 (LD50),生长,繁殖和人口动态.
对氧化应激标记物 (ROS,MDA) 和抗氧化酶活性进行生物化学分析.
对DNA损伤的分子分析和DNA修复基因的表达.

主要成果:

罗提弗表现出对UV-B的耐受性 (LD50 = 28.53 kJ/m2),但经历了增长和繁殖的减少.
观察到显著的氧化损伤 (增加ROS,MDA) 和脂质过氧化.
抗氧化酶活性是剂量依赖的,在低剂量UV-B时增加,在高剂量时减少.
紫外线B诱导的DNA损伤和剂量依赖的DNA修复基因的上调.

结论:

布拉奇乌斯 (Brachionus asplanchnoidis) 对紫外线B的反应复杂,包括耐受性,生理障碍和分子防御激活.
该研究提供了对水生无脊椎动物UV-B压力背后的生态和分子机制的见解.
评估UV-B的影响需要考虑生物体的健康和分子修复过程.

关键词:

B. 阿斯普兰科尼迪斯造成的DNA损伤是DNA损伤.生命史参数生命史参数氧化应激是一种氧化应激.人口增长率的人口增长率.紫外线-B辐射的辐射

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