在含有Cu (II) 的尿素上丰富生物肥料类型的氧化细菌
Yi-Xin Zhang1, Yong-Xin Li1, Wei Zhang2
1Centre of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Environmental research
|August 5, 2023
概括
这项研究使用尿素作为肥料成功丰富了氧化细菌 (HOB). 铜抑制了HOB的生长,但产生了具有生物肥料潜力的耐铜菌株.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 农业科学 农业科学
背景情况:
- 化学肥料如尿素有助于通过和重金属对环境的污染.
- 生物肥料为化学肥料提供了可持续的替代品,提高了作物产量并减少了生态影响.
- 氧化细菌 (HOB) 作为生物肥料具有前景,但它们使用尿素进行丰富和对铜等重金属的耐受性需要研究.
研究的目的:
- 用尿素作为唯一的源来丰富氧化细菌 (HOB).
- 研究铜 (Cu2+) 对HOB丰富,生长和活性的影响.
- 在不同的条件下评估HOB社区的生物肥料潜力 (ACC降解和酸盐溶解).
主要方法:
- 在受控环境中使用尿素丰富HOB.
- 微生物社区分析以确定主导细菌物种.
- 测量尿素转化率和HOB增长.
- 细胞外聚合物物质 (EPS) 的分析,包括多糖和蛋白质含量.
- 对ACC降解和酸盐溶解能力的评估.
主要成果:
- 使用尿素成功丰富HOB,达到平均尿素转化率 (AUCR) 为180.08 mgN/L/d,由Hydrogenophaga主导.
- 添加5.47 mg/L Cu2+抑制了HOB增长48%,并减少了AUCR16%,达到151.18 mgN/L/d.
- 铜暴露增加了EPS多糖和蛋白质含量,表明应激反应.
- 在铜的存在下,微生物群落转向了Azospiralium的主导地位 (91.33%).
- 两个丰富的HOB社区都展示了ACC降解和酸盐溶解能力.
结论:
- 尿素可以有效地用于直接丰富生物肥料类型HOB.
- 耐铜的HOB菌株可以从含有尿素的废水中获得,含量为5.47 mg/L Cu2+.
- 这些发现表明,使用HOB.使用铜污染的工业废水生产生物肥料的可行方法.
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