对潜在有毒元素的职业暴露改变了正式和非正式的巴西工人的基因表达特征
Fernanda Junqueira Salles1, Ilias S Frydas2, Nafsika Papaioannou2
1Department of Environmental Health, School of Public Health, University of Sao Paulo, Av. Dr. Arnaldo, 715, Cerqueira Cesar, CEP 01246-904, São Paulo, SP, Brazil; The Human Exposome Research Group/ Expossoma e Saúde do Trabalhador - eXsat, School of Public Health, University of Sao Paulo, Av. Dr. Arnaldo, 715, Cerqueira César, Sao Paulo, SP, 01246-000, Brazil.
Environmental research
|August 5, 2023
概括
职业接触有毒金属会改变巴西工人的基因表达,主要影响炎症和免疫反应. 这表明与慢性病和癌症的潜在联系,需要进一步调查.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 分子生物学分子生物学
背景情况:
- 众所周知,包括有毒金属在内的化学元素会影响基因表达.
- 巴西的职业暴露带来了各种环境健康挑战.
研究的目的:
- 将非正式珠宝工人和正式钢铁工业工人的基因表达特征与对照组进行比较.
- 确定受巴西不同职业暴露影响的分子通路.
主要方法:
- 微阵列分析了68个来自非正规劳动者,正规劳动者和对照者的血液样本.
- 使用Agilent平台和GeneSpring软件进行基因表达分析.
- 统计和途径分析以确定差异表达的基因和丰富的途径.
主要成果:
- 非正规工作者表现出16个上调和33个下调基因,富含MAPK,Toll-like受体和NF-kappa B信号通路 (炎症/免疫反应).
- 正式工作者表现出20个上调和50个下调的基因,这些基因与抗微生物,中性粒细胞脱粒和动脉样硬化途径有关.
- 在这两组工人中,IFI27基因的表达常见差异化,并且与癌症有关.
结论:
- 职业暴露显著改变基因表达,主要影响炎症和免疫反应途径.
- 观察到的基因表达变化表明,长时间接触金属与慢性疾病 (包括心血管疾病和癌症) 之间存在潜在联系.
- 需要进一步的研究来验证因果关系,并调查这些暴露对健康的影响.
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