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一个Chicken Tapasin ortholog可以陪伴空的HLA-B37:01分子,独立于其他载荷组件

Georgia F Papadaki¹, Claire H Woodward¹, Michael C Young¹

¹Center for Computational and Genomic Medicine, Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

The Journal of biological chemistry

|August 5, 2023

概括

肉塔帕辛 (chTapasin) 是一种稳定,重组蛋白质,可以结合人类的MHC-I分子. 这种稳定的支架为人类MHC-I和MHC类分子提供了载和蛋白质工程.

科学领域:

免疫学免疫学免疫学
结构生物学结构生物学
蛋白质工程是指蛋白质工程.

背景情况:

人类塔帕辛 (hTapasin) 对于MHC-I加载至关重要,但在重组后是不稳定的.
重组hTapasin需要像ERp57这样的辅助因子进行体外功能,限制了应用.
由于hTapasin的不稳定性,生成特定的MHC-I (pMHC-I) 分子具有挑战性.

研究的目的:

为了研究Tapasin (chTapasin) 作为hTapasin的稳定,重组替代品的潜力.
描述chTapasin和人类MHC-I分子之间的相互作用.
探索chTapasin在蛋白质工程中对于pMHC-I生成的实用性.

主要方法:

对chTapasin的重组表达.
使用基于甲基的NMR进行生物物理表征.
对chTapasin与人类HLA-B*37:01.01结合的分析
对chTapasin-HLA复合体的受性的评估.

主要成果:

在高收益率下重组表达的chTapasin是稳定的,并且没有共同chaperones.
chTapasin与人类的HLA-B*37:01.01形成了一个稳定的三级复合体.
核磁共振研究表明,chTapasin在HLA-B*37:01.01上识别了一个保存的β2m表位.
B*37:01/ch塔帕辛复合体是受体,在高 afinity 结合时分离.

结论:

chTapasin 作为蛋白质工程应用的稳定支架.
它扩大了人类MHC-I和MHC类分子上的联结体交换功能.
chTapasin为产生具有所需抗原特异性的pMHC-I分子提供了一个有前途的工具.

关键词:

塔帕辛 (Tapasin) 是一种水.抗原的处理和呈现方式.主要组织相容性复合体 (MHC)分子陪伴者分子陪伴者蛋白质的奥斯托洛格.

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