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用UHPLC-MS/MS基于其特征性的基础上,对乳制品中的氨酸糖马克罗的表征和确定

Qin Chen¹, Shiyun Lai², Lezhen Dong¹

¹State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, School of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang, PR China.

|August 6, 2023

概括

一种新的UHPLC-MS方法准确量化了乳制品中的氨酸糖巨 (CGMP). 该研究确定了MAIPPK为在牛产品中检测CGMP的可靠标记物.

科学领域:

分析化学分析化学
食品科学食品科学食品科学
蛋白质组学是指蛋白质组学.

背景情况:

对乳制品分析来说,精确量化素糖分巨 (CGMP) 是非常重要的.
现有的方法可能面临着对矩阵效应和特异性的挑战.

研究的目的:

开发和验证一种超高性能液体染色体协同质谱法 (UHPLC-MS) 方法,用于精确的CGMP量化.
识别和使用一种特征性来可靠地检测牛奶产品中的CGMP.

主要方法:

使用UHPLC-MS.的有针对性的蛋白质组学方法.
高分辨率质谱仪 (HRMS) 用于定性分析.
以同位素标记的来纠正矩阵效应.
素消化以确定特征性MAIPPK.

主要成果:

优化预处理条件,包括氧化剂和酸度以及氧化时间.
方法验证显示恢复率为103.7%,精度低于5% (白天和白天).
MAIPPK 已被证实是从 κ-casein 获得的 CGMP 的代表性标记物.

结论:

开发的UHPLC-MS方法提供了乳制品中CGMP的准确和精确量化.
特征性MAIPPK是一种适合于检测CGMP在各种牛乳母体中的量化标志物.

关键词:

卡赛因糖分巨是一种糖分巨 (casein glycomacropeptide).具有特征性的.高分辨率质谱仪 (HRMS) 是一种高分辨率的质谱仪.有针对性的蛋白质组学.超高性能液体色谱 (UHPLC) 是一种超高性能液体色谱.

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