对比嵌入的聚乙烯胺) 嵌入的黄油和基于八酸的色谱静止相的分离性能
Yaxin Yang1, Jingqiu Zhou1, Qiaojie Liang1
1School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.
Journal of chromatography. A
|August 6, 2023
概括
两种新的静止相,Sil-PEI-BAD和Sil-PEI-CAD,是由聚乙烯胺 (PEI) 修改的球合成的. 这些相表现出水友性和疏水性化合物的独特色谱性能,受链长度的影响.
科学领域:
- 材料科学 材料科学 材料科学
- 染色体学 染色体学 是一种染色学.
- 表面化学 表面化学
背景情况:
- 球被广泛用于染色学中的静止相.
- 用聚合物如聚乙烯胺 (PEI) 修改表面可以改变它们的染色学特性.
- 将不同的功能组植入改性上可以为特定的分离创建定制的静止相.
研究的目的:
- 通过将黄油酸和酸接种在PEI修饰的球上,合成和描述两种新型静止相,Sil-PEI-BAD和Sil-PEI-CAD.
- 为了评估这些新的静止相的染色学性能,用于分离各种分析物.
- 研究基链长度对保留机制和分离能力的影响.
主要方法:
- 合成PEI修饰的二氧化球.
- 将黄油酸 (Sil-PEI-BAD) 和八酸 (Sil-PEI-CAD) 植入改性上.
- 使用里埃变换红外光谱 (FT-IR),元素分析 (EA) 和热重力测量分析 (TGA) 的表征.
- 使用水友性 (核化物,类,维生素) 和水性 (PAH,基) 化合物进行色谱评估.
主要成果:
- 通过FT-IR,EA和TGA证实Sil-PEI-BAD和Sil-PEI-CAD的成功合成.
- 西尔-PEI-BAD证明了水友性化合物的优越分离.
- Sil-PEI-CAD显示出水化合物,包括PAHs和基的良好分离.
- Sil-PEI-CAD显示出基于分离的HILIC/RPLC混合模式染色学的潜力.
- 分离性能与移植的基链的长度直接相关.
结论:
- 在PEI修饰的球上移植的链的长度显著影响了静止相的特性和保留机制.
- Sil-PEI-BAD和Sil-PEI-CAD分别为水友性和水害性分析物提供了不同的和互补的分离能力.
- 这些新的静止相为色谱分离提供了多功能选择,Sil-PEI-CAD对混合模式色谱显得有希望.
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