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牛乳腺炎病原体对抗微生物敏感性测试的汁微稀和阿加盘扩散方法之间的比较

Giorgia Palladini¹, Chiara Garbarino¹, Andrea Luppi¹

¹Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), Strada Faggiola 1, 29027-Gariga di Podenzano, (PC), Italy.

Journal of microbiological methods

|August 6, 2023

概括

在对牛乳腺炎的抗微生物敏感性测试进行比较时,汁微稀释 (BMD) 和亚格盘扩散 (ADD) 方法显示出相当的总体一致. 虽然BMD更为严格,但这两种方法对于指导牛的抗生素使用有价值.

科学领域:

兽医微生物学兽医微生物学
抗微生物耐药性抗微生物耐药性
牛的健康问题

背景情况:

抗生素耐药性需要在牲畜中合理使用抗菌剂,特别是用于治疗牛乳炎,这是牛类抗生素消费的主要驱动因素.
准确的抗微生物敏感性测试对于选择有效的治疗方法和打击耐药性至关重要.

研究的目的:

为了比较使用Sensititre定制板的微稀释 (BMD) 方法与牛乳腺炎病原体的亚格盘扩散 (ADD) 方法的准确性和一致性.
评估BMD和ADD之间的协议,针对各种针对常见的传染性和环境乳腺炎引起细菌的抗微生物药物.

主要方法:

评估了215个牛乳腺炎分离物,包括金黄色葡萄球菌,Streptococcus agalactiae,Streptococcus uberis,大肠杆菌等.
对12种抗微生物药物的ADD结果与Sensititre定制板的BMD结果进行了比较.
应用于MIC和区域直径的CLSI和EUCAST断点,分析差异 (VMD,MD,MiD).

主要成果:

骨和ADD之间的总体一致性为80.7% (科恩卡帕=0.397),表明相当一致.
对于黄金葡萄球菌和马氏状菌 (Serratia marcescens) 发现了令人满意的协议 (89-100%).
对于特定的病原体-抗微生物组合,如Streptococcus uberis与里芬素 (20%) 和大肠杆菌与安培西林 (20%) 等,观察到较低的一致性.

结论:

牛乳炎病原体的BMD和ADD方法显示相当一致,BMD通常更为限制性.
差异凸显了需要特定于方法或特定于病原体的断点,以优化抗微生物选择.
尽量减少非常大的差异 (0.2%) 对于防止牛乳腺炎病例的治疗失败至关重要.

关键词:

阿加尔盘的扩散是阿加尔盘的扩散.抗微生物耐药性抗微生物耐药性牛的乳腺炎可能是牛乳炎.苏微稀释的微稀释方法

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