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合成微生物群落的反应及其与聚乙烯纳米塑料在微生物电解细胞中的相互作用
Basem S Zakaria1, Seyed Mohammad Mirsoleimani Azizi1, Biplob Kumar Pramanik2
1Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, Canada.
The Science of the total environment
|August 6, 2023
概括
聚烯纳米塑料 (PsNPs) 在500μg/L显著抑制微生物电解细胞,减少电流和的生产. 这项研究确定了电活性生物膜中纳米塑料的抑制值.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 电化学 电化学 电化学
背景情况:
- 微生物电化学技术 (MET) 提供了能源回收和废水处理的双重好处.
- 像微/纳米塑料 (MP/NP) 这样的新兴污染物对废水系统构成风险,但它们对MET的影响还未得到充分研究.
- 纳米塑料比微塑料小,由于表面积和透潜力增加,可能对微生物群体产生更深远的影响.
研究的目的:
- 调查聚乙烯纳米塑料 (PsNPs) 对微生物电解细胞 (MEC) 中的合成阳极微生物群落的影响.
- 确定在MEC的关键性能指标和微生物社区结构上PsNP的抑制值.
主要方法:
- 在MEC中对不同度的PsNPs (50,250和500μg/L) 暴露的合成阳极微生物群落的暴露.
- 监测当前密度和气生产速度.
- 细胞外聚合物质 (EPS) 组成的分析.
- 基因表达分析 (细胞外电子转移基因的下调).
- 使用16S rRNA基因测序进行微生物社区分析,以评估关键细菌的多样性,丰富性和丰富性.
主要成果:
- 低度的PsNP (50和250μg/L) 的影响是最小的.
- 500μg/L的PsNP显著降低了最大电流密度 (~43%) 和特定生成率 (~48%).
- 暴露于PsNP增加了EPS水平,改变了碳水化合物与蛋白质的比例,这可能表明了防御反应.
- 基因表达分析显示,在500μg/L的PsNP时,细胞外电子转移基因的下调.
- 微生物分析显示,16S rRNA基因拷贝减少,主要电活性和发酵细菌的多样性,丰富性和丰富性在500μg/L的PsNPs下降.
结论:
- 这项研究提供了METs中合成电活性生物膜上纳米塑料的第一个报告的抑制值.
- 500μg/L的聚烯纳米塑料对MEC性能和微生物群落结构产生不利影响.
- 了解纳米塑料的影响对于MET在废水处理和能源回收方面的有效应用至关重要.
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