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登革热病毒血清型 2 基因型 III 演变在巴西中西部马托格罗索州2019年爆发期间

Marcelo Adriano Mendes Dos Santos¹, Janeth Aracely Ramirez Pavon², Lucas Silva Dias³

¹Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil; Faculdade de Medicina, Universidade do Estado de Mato Grosso, Cáceres, MT, Brazil.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases

|August 6, 2023

概括

登革热病毒2型 (DENV-2) 基因型III,血统BR4,在巴西马托格罗索州的2019年爆发期间显示出显著的演变. 基因组监测对于跟踪可能与免疫逃避和未来疫情相关的病毒变化至关重要.

科学领域:

病毒学病毒学
基因组学就是基因组学.
流行病学流行病学

背景情况:

2019年,巴西的登革热发病率升了70%,主要是由2型登革热病毒 (DENV-2) 推动的.
在此期间,马托格罗索州 (MT) 经历了相当大的树冠病毒爆发.

研究的目的:

调查DENV-2的基因组特征和演变在MT的2019年爆发期间.
为了确定DENV-2菌株中的新突变和进化模式.

主要方法:

使用Illumina NextSeq对92名怀疑感染 arbovirus 的患者血清样本进行基因组学测序.
分析完整和近乎完整的病毒基因组,包括DENV-2,人类帕沃病毒B19和Coxsackievirus A6.
遗传学分析以确定DENV-2血统并识别独特的突变.

主要成果:

确定了八个完整的和两个近乎完整的DENV-2基因型III基因组 (BR4谱系).
在DENV-2株的E,NS3和NS5蛋白中发现了独特的非同义突变.
在DENV-2基因型III中发现了几种以前未报告的氨基酸变化,包括E蛋白的ectodomain和NS5甲基转移酶域的突变.

结论:

该研究强调了2019年MT爆发期间DENV-2基因型III BR4血统中的显著演变.
基因组监测对于检测病毒进化,潜在的免疫逃避和病毒健康状况的变化至关重要.
这些发现强调了持续基因组监测的重要性,以预测和管理未来的登革热爆发.

关键词:

登革热病毒 2 登革热病毒转基因组学是指转基因组学.病毒遗传学病毒遗传学病毒的演化病毒的演化

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