在蓝色细菌培养中表征胡卜素 - - 对古生物学研究的机遇和影响
Jonathan Puddick1, Sebastian Naeher2, John K Pearman1
1Cawthron Institute, 98 Halifax Street-East, Nelson 7010, New Zealand.
Harmful algae
|August 6, 2023
概括
湖泊沉积物中的蓝藻细菌花色素可以揭示过去的生态系统变化. 特定的胡卜素比率,比如芝丁/埃奇宁,显示出跟踪蓝菌种群历史变化的潜力.
科学领域:
- 环境科学 环境科学
- 古代气候学是一种古气候学.
- 微生物学 微生物学
背景情况:
- 全球日益增多的蓝菌繁殖威胁着水生生态系统和人类健康.
- 湖泊沉积物中的蓝藻细菌颜料提供了对历史开花动态的见解.
- 关于胡卜素对某些类型的蓝藻细菌的特异性存在不确定性.
研究的目的:
- 为了研究在培养菌株中蓝藻细菌胡卜素的分布.
- 在古生物学研究中评估胡卜素度和比率作为蓝藻细菌生物质和社区组成的代理值的实用性.
- 将这些发现应用于新西兰湖泊沉积物核心,以重建历史的蓝藻细菌群落.
主要方法:
- 在34种蓝菌培养中对颜料 (胡卜素) 的分析.
- 特定的胡卜素的量化:坎塔桑丁,埃希诺,泽亚桑丁和myxoxanthophyll.
- 颜色分析和16S rRNA基因元编码对来自新西兰三个湖泊的沉积物核心的应用.
主要成果:
- 康塔克桑丁,埃希宁和泽亚桑丁是无处不在的;myxoxanthophyll仅限于特定的属 (Microcoleus,Planktothrix) 和皮科亚诺细菌.
- 胡卜素度的总和与蓝藻细菌生物量 (R2 = 0.58) 相对相关性很好,这对于一般丰度估计很有用.
- 胡卜素的比率,特别是泽亚桑/埃希宁,区分皮科亚诺细菌 (高比率) 和形成花的蓝藻细菌 (低比率),在培养和沉积芯之间是一致的.
结论:
- 蓝藻细菌的胡卜素度可以估计历史的蓝藻细菌生物量.
- 泽亚桑/埃希宁的比率显示,作为一个古生态学代理区分皮可西亚诺细菌和开花形成的蓝藻细菌的承诺.
- 需要进一步的研究来验证山丁/乙诺因比率用于重建过去的蓝藻菌群体结构.
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