相关实验视频

Updated: Jul 20, 2025

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

Published on: July 3, 2015

在体内,阿斯塔山丁对抗电离辐射诱导的DNA损伤的保护性有效性

Dilek Aşcı Çelik¹, Vehbi Atahan Toğay¹

¹Süleyman Demirel University, Faculty of Medicine, Department of Medical Biology, Isparta, Turkey.

Chemical biology & drug design

|August 6, 2023

概括

阿斯塔丁补充剂显著减少了大鼠辐射诱导的DNA损伤. 这种胡卜素在保护DNA免受电离辐射损伤方面表现有前途.

科学领域:

生物化学生物化学
放射学放射学是一门学科.
药理学药理学是指药理学的学科.

背景情况:

阿斯塔丁是一种天然的胡卜素颜料,具有抗氧化特性.
在放射治疗和其他暴露中,DNA损伤是一个关键问题.
以前的研究表明,阿斯塔克桑在DNA修复机制中的潜力.

研究的目的:

评估阿斯塔克桑丁在减轻大鼠全身放射治疗引起的DNA损伤方面的疗效.
为了研究阿斯塔克桑丁对电离辐射暴露的保护作用.

主要方法:

32只雄性大鼠被分为四组:对照组,电离辐射组,阿斯塔克桑丁组和辐射+阿斯塔克桑丁组.
辐射组接受了8个灰色的X射线暴露.
阿斯塔克桑丁 (4毫克/公斤) 被口服7天,从照射开始.
使用彗星分析量化DNA损伤,特别是测量%TailDNA参数.

主要成果:

与对照组和仅服用阿斯塔山丁的组相比,电离辐射显著增加了DNA损伤 (%TailDNA) (p < .001).
与仅接受辐射的组相比,在辐射+阿斯塔克桑组中,阿斯塔克桑的使用导致DNA损伤显著减少 (p < .05).
虽然阿斯塔山丁可以减少辐射引起的损伤,但它不能完全恢复DNA完整性到控制水平.

结论:

施用阿斯塔丁在老鼠中显示出对辐射诱导的DNA损伤有显著的保护作用.
这些发现支持了阿斯塔克桑是减少DNA损伤的有希望的药物这一假设.
需要进一步的研究来探索阿斯塔克桑在减轻辐射诱导的基因毒性方面的治疗潜力.

关键词:

胡卜素是一种胡卜素.彗星测试试验彗星测试试验遗传毒性遗传毒性遗传毒性辐射疗法辐射疗法它们是大鼠淋巴细胞.

更多相关视频

Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition

Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition

Published on: November 14, 2013

Quantitation of γH2AX Foci in Tissue Samples

Quantitation of γH2AX Foci in Tissue Samples

Published on: June 28, 2010

相关概念视频

Mutations

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...

Biological Effects of Radiation

Biological Effects of Radiation

All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...

Nucleotide Excision Repair

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...