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对肝细胞癌术后复发的关键长非编码RNA和mRNA表达特征的转录组测序分析

Xiaohui Yao^1,2,1, Shan Liu^3,1, Huan Xia^4,1

¹The Research Center of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

Technology and health care : official journal of the European Society for Engineering and Medicine

|August 7, 2023

概括

增加像XLOC_063499和XLOC_042016这样的长非编码RNAs (lncRNAs) 可能通过调节脂质代谢途径来防止肝细胞癌 (HCC) 复发. 这些发现为lncRNA提供了新的见解.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

手术后复发是肝细胞癌 (HCC) 患者的主要死亡原因.
长非编码RNAs (lncRNAs) 在HCC进展和预后中的作用是公认的,但它们在术后复发中的具体参与需要进一步的系统调查.

研究的目的:

系统地识别肝切除后肝细胞癌 (HCC) 复发所涉及的关键长非编码RNA (lncRNA) 和它们的目标信使RNA (mRNA).
探索与HCC复发中差异表达的lncRNA和mRNA相关的功能途径和调节网络.

主要方法:

使用RNA-sequencing (RNA-seq) 来分析在正型HCC模型和复发性HCC模型中的lncRNA和mRNA的转录组表达特征.
定量实时聚合酶链反应 (qRT-PCR) 用于验证差异表达的lncRNAs (DElncRNAs) 和它们的目标mRNAs的表达水平.
进行了生物信息分析,包括基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富,以及网络构建 (lncRNA-mRNA相互作用,蛋白质-蛋白质相互作用).

主要成果:

发现大量的lncRNAs (211) 和mRNAs (1125) 在两组之间表达不同.
丰富分析表明,这些差异表达的基因主要参与脂质代谢途径,如阿拉基酸代谢和PPAR信号传递.
特定的DElncRNAs (XLOC_063499,XLOC_042016) 被确定为可能通过在脂质代谢途径内调节目标细胞色素P450 (CYP) 家族基因来预防HCC复发.

结论:

这项研究为在HCC复发的背景下 lncRNAs 的生物功能提供了新的见解.
已识别的DElncRNA及其相关的脂质代谢途径为预防手术后HCC复发提供了潜在的目标.

关键词:

在此之前,CYP CYP HCC的复发情况这是一个LncRNARNA.在RNA-seqqq.肝瘤术后复发模型手术后发生的HCC复发.

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