在聚电解质三元复合体中封装和稳定乳酸铁素
Tiantian Lin1, Yufeng Zhou1, Younas Dadmohammadi1
1Department of Food Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA.
Food hydrocolloids
|August 7, 2023
概括
新型聚电解质三元复合物增强乳酸铁素 (LF) 的稳定性和抗菌活性. 阿拉伯-LF-凝复合体表现出异常的耐热性,保留了用于食品应用的LF功能.
科学领域:
- 食品科学 食品科学 食品科学
- 生物材料科学 生物材料科学
- 蛋白质化学 蛋白质化学
背景情况:
- 乳酸 (LF) 是一种具有宝贵性质的生物活性蛋白质,但容易因热量和环境因素而变质.
- 这种不稳定性限制了其在功能性食品中的应用,需要改进交付和稳定策略.
研究的目的:
- 开发新型的多电解质三元复合物,以增强乳酸铁素 (LF) 的热稳定性并保持其抗菌活性.
- 研究这些复合体的结构-属性关系,特别是聚焦于多相协体.
主要方法:
- 使用LF,凝 (G) 和负电荷的多糖体 (阿拉伯,大豆可溶性多糖体,高甲氧丁) 制备三元复合物.
- 复杂结构的表征,包括聚合物间复合物和同体 (观察多相同体).
- 通过热处理 (90°C2分钟,145°C30秒) 评估热稳定性,并评估保留的LF结构和抗菌活性.
主要成果:
- 三级复合物,特别是含有阿拉伯糖 (GA),大豆可溶性多糖化物 (SSP) 和高甲氧丁 (HMP),显著改善了LF的热稳定性.
- 该GA-LF-G复合体表现出了显著的稳定性,在严重的热处理后保留了90%以上的本地LF,而对LF控制则为7%左右.
- 在三元复合体内的LF在热处理后保持了对格拉姆阳性和格拉姆阴性细菌的显著抗菌活性.
结论:
- 聚电解质三元复合物有效地增强了乳酸的热稳定性并保持了其生物活性,克服了功能性食品应用中的局限性.
- 观察到的多相协体结构,特别是在GA-LF-G复合体中,显示出作为封装和稳定其他敏感生物活性和的多功能模板的潜力.
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