在森林和田野中Panax notoginseng中使用高性能液态染色学确定丹西
Chao Ji1, Yuxiao Lu2, Juan Li1
1Laboratory for Quality Control and Traceability of Food and Agricultural Products, Tianjin Normal University, Tianjin 300387, China.
ACS omega
|August 7, 2023
概括
在Panax notoginseng中的丹西辛积累量因植物部分和种植方式而异. 这项研究提供了一种量化登西的方法,有助于对这种有价值的成分进行质量控制.
科学领域:
- 药理学是指药理学,即药理学是指药理学.
- 分析化学 分析化学
- 植物科学 植物科学
背景情况:
- 丹西辛是Panax notoginseng中关键的非蛋白质氨基酸,具有静血和抗炎性质.
- 有限的研究存在于影响dencichine积累的因素,如地理来源和种植方法.
研究的目的:
- 来自云南,中国,一个主要的种植和贸易中心的Panax notoginseng中的dentichine含量量.
- 调查不同植物部位,种植环境 (森林与田野),起源和高度对素积累的影响.
- 为了验证一个优化的分析方法来确定dentichine.
主要方法:
- 使用二极管阵列探测器的优化高性能液态染色学 (HPLC) 方法,用于在没有衍生化的情况下量化基因.
- 分析了Panax notoginseng的640多个样本.
- 方法验证包括恢复率 (80-110%) 和相对标准偏差 (<10%).
主要成果:
- 植物各部分的丹西含量有显著差异:幼根 (39.59%) >主要根 (29.91%) >叶子 (16.21%) >茎 (14.29%).
- 叶子素水平在林业种植中较高 (5.52 ± 2.26 mg/g),而不是田间种植 (3.93 ± 1.72 mg/g),主要根的反向关系.
- 原产地和海拔高度显然影响了基因的积累.
结论:
- 开发的HPLC方法是有效的确定dencichine在各种Panax notoginseng部分和来源.
- 这些发现为Panax notoginseng的质量控制和基于dencichine的产品的开发提供了关键数据.
- 种植方式和地理因素对Panax notoginseng的植物化学特征产生重大影响.
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