在低pH值下陶马丁的热降解及其使用基酸盐的预防
Benjamin Pomon1, Yu Zhao1, Alex L Lai2
1Department of Food Science, College of Agriculture and Life sciences, Cornell University, Stocking Hall, Ithaca, NY, 14853.
Food hydrocolloids
|August 7, 2023
概括
陶马丁是一种天然的甜味剂,由于蛋白质的聚合和碎片化,在加热时失去甜味. 酸性条件减缓了这一过程,而基酸防止了基质损伤,保持了甜味.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物化学 生物化学
背景情况:
- 来自Katemfe工厂的天然甜味剂thaumatin是人工甜味剂的潜在替代品.
- 在热加工过程中的稳定性对于食品工业应用至关重要.
- 了解陶马丁的降解途径对于其有效使用至关重要.
研究的目的:
- 为了研究陶马丁在热处理过程中的稳定性.
- 为了阐明陶马丁在加热后甜度丧失背后的机制.
- 确定保存陶马丁功能的方法.
主要方法:
- 在不同的pH值条件下加热thaumatin (中性,基性,酸性).
- 使用SDS-PAGE.评估甜度损失和蛋白质聚合.
- 使用脂友性旋转陷BEMPO-3检测自由基.
- 测量蛋白质碳基含量作为氧化指标.
- 评估基酸盐的抑制作用.
主要成果:
- 陶马丁在加热时失去甜味,特别是在中性/基本条件下,由于通过二硫化物键交换的聚合.
- 酸性pH提供了更大的抵抗甜味的损失,虽然碎片化发生.
- 加热会诱导自由基的产生和蛋白质的氧化 (碳基的形成).
- 基甲酸有效地抑制了激素的形成和蛋白质碳基的增加.
结论:
- 陶马丁的甜味因热处理而受到损害,通过聚合和激素介导的降解.
- 酸性条件提供部分保护,但激素通路有助于不稳定.
- 基甲酸显示出作为一种保护剂的潜力,可以防止陶马丁的热降解.
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