代谢重组控制了微生物对温度变化的内在反应
Benjamin D Knapp1, Lisa Willis2, Carlos Gonzalez3
1Biophysics Program, Stanford University, Stanford, CA 94305, USA.
bioRxiv : the preprint server for biology
|August 7, 2023
概括
细菌对温度变化表现出一种保存的,渐进的反应,由一种新的温度记忆机制驱动. 这一发现解释了微生物在不同温度和营养条件下的生长动态.
科学领域:
- 微生物生理学 微生物生理学
- 热力学是一种热力学.
- 生物化学 生化学
背景情况:
- 微生物的行为取决于温度,但在中间温度下控制生长的机制尚不清楚.
- 现有的研究集中在极端的热/冷冲击上,忽视了逐渐的温度反应.
- 了解这些反应对于预测各种环境中的微生物动态至关重要.
研究的目的:
- 阐明细菌对温度上升的逐渐反应背后的机制.
- 为了确定微生物细胞中温度记忆的性质.
- 开发一种解释温度依赖微生物生长和适应的模型.
主要方法:
- 在受控温度扰动期间的单细胞显微镜.
- 研究转录,转化和膜组件.
- 开发和测试基于酶动力学和代谢组重组的计算模型.
主要成果:
- 细菌对温度上升的反应时间保持稳定,大约是1.5倍,独立于初始/最终温度或营养.
- 发现了一种新的温度记忆,不依赖于转录,翻译或膜变化.
- 一个具有温度敏感动态的自催化酶网络模型在各种条件下成功地总结了观察到的动态和预测的反应.
结论:
- 这项研究阐明了温度依赖微生物生长的机制基础.
- 代谢组重组机制编码温度记忆,影响细胞适应.
- 这些发现为理解Arrhenius依赖生长和Monod动力学提供了一个框架.
相关概念视频
01:27Factors Influencing Microbial Growth: Temperature
67
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
67
01:29Diversity of Archaea IV
51
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
51
02:45Responses to Heat and Cold Stress
13.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.6K
01:30Other Stress Responses in Bacteria
33
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
33
01:23Physical Methods for Controlling Microbial Growth: Temperature
49
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
49
02:28Effect of Temperature Change on Reaction Rate
4.2K
The Arrhenius equation,
4.2K