Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Splicing01:32

RNA Splicing

56.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.5K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.3K
5.3K
Alternative RNA Splicing02:18

Alternative RNA Splicing

3.8K
3.8K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

2.7K
2.7K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.5K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.5K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Efficient evidence-based genome annotation with EviAnn.

Nature methods·2026
Same author

Testing the reliability of AI-generated protein structures.

bioRxiv : the preprint server for biology·2026
Same author

Integrating Negative-Pressure Wound Therapy in the Therapeutic Protocol of Extensive Pediatric Burns: Current Practice and Further Treatment Decision Algorithm.

Medicina (Kaunas, Lithuania)·2026
Same author

Mesenchymal Stem Cell-Derived Extracellular Vesicles and Plant-Derived Nanovesicles as Cell-Free Therapies for Thermal Burn Healing: A Systematic Review of Preclinical Evidence and Delivery Strategies.

Medical sciences (Basel, Switzerland)·2026
Same author

StringTie3 improves total RNA-seq assembly by resolving nascent and mature transcripts.

Nature methods·2026
Same author

Comparison of unbiased metagenomic next generation sequencing to targeted multiplex diagnostic assays for the detection of respiratory viruses.

PloS one·2026

相关实验视频

Updated: Jul 19, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.8K

斯普拉姆:基于深度学习的拼接位置预测器,可以改善拼接对齐.

Kuan-Hao Chao1,2, Alan Mao1,2,3, Steven L Salzberg1,2,3,4

  • 1Department of Computer Science, Johns Hopkins University, Baltimore, MD 21218, USA.

bioRxiv : the preprint server for biology
|August 7, 2023
PubMed
概括

一种新的深度学习方法Splam通过分析侧面序列和对捐赠者-接受者对进行训练,准确地预测DNA中的拼接接口. 这种方法改善了RNA测序数据分析和基因变异识别.

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

更多相关视频

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.8K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.7K

相关实验视频

Last Updated: Jul 19, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.8K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.8K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.7K

背景情况:

  • RNA拼接对于基因表达和创建基因变异至关重要.
  • 准确的拼接连接预测对于理解基因功能和疾病至关重要.

结论:

  • 斯普拉姆提供了一种更快,更准确的方法来检测拼接连接.
  • 在RNA-seq数据中清理错误的拼接对齐提供了可靠的解决方案.
  • 增强下游转录基因组分析,用于poly (A) 和ribos-depleted库.