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功能化的石墨烯氧化物网格使SNF2h核体复合体的高分辨率冷EM结构能够在没有交叉连接的情况下实现

Un Seng Chio¹, Eugene Palovcak², Anton A A Autzen^3,4

¹Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA.

bioRxiv : the preprint server for biology

|August 7, 2023

概括

新的石墨烯氧化物EM网格防止变质,使SNF2h等酶核酶体复合物的高分辨率冷EM成为可能. 这一突破改善了染色体重塑剂和其他蛋白质与核酸相互作用的结构分析.

科学领域:

结构生物学结构生物学
生物化学生物化学
分子生物物理学分子生物物理学

背景情况:

低温电子显微镜 (cryo-EM) 对于确定酶核细胞组复杂结构至关重要.
用于冷-EM的样本准备经常受到空气-水接口 (AWI) 的变性阻碍.
现有的方法难以保持微妙的蛋白质核酸复合物的完整性.

结论:

开发的EM网格有效地保护酶核酶组复合体免受AWI变性.
这种方法促进了对染色体重塑剂和其他蛋白质核酸复合物的详细结构研究.
该方法有望促进分子机制的结构生物学研究.

关键词:

在ISWIWI中使用.这就是SNF2h.染色体改造器染色体改造器石墨烯氧化物石墨烯氧化物核子组中的核子单颗粒冷电磁体

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相关概念视频

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...