相关实验视频

Updated: Jul 19, 2025

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

15.2K

蛋白质氨酸酸酶中的Allostery是由不同的动力学启用

Colin L Welsh, Lalima K Madan

    bioRxiv : the preprint server for biology
    |August 7, 2023
    PubMed
    概括

    蛋白质超级家族中的微妙序列变化改变了蛋白质的动态,而不是结构,影响了酶的催化能力. 这项研究揭示了蛋白质动力学如何解释相关酶之间的催化效率和全控制的变化.

    更多相关视频

    Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases06:56

    Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

    Published on: September 6, 2024

    415
    A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors10:17

    A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

    Published on: April 29, 2022

    2.5K

    相关概念视频

    Protein Kinases and Phosphatases02:54

    Protein Kinases and Phosphatases

    Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
    Protein kinases
    Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
    13.2K
    Allosteric Proteins-ATCase01:19

    Allosteric Proteins-ATCase

    Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
    Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
    5.8K
    Cooperative Allosteric Transitions01:58

    Cooperative Allosteric Transitions

    2.3K
    Assembly of Signaling Complexes01:30

    Assembly of Signaling Complexes

    Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
    Interaction domains in cell signaling
    Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
    5.8K
    Ligand Binding and Linkage00:49

    Ligand Binding and Linkage

    Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
    4.8K
    Phosphorylation01:02

    Phosphorylation

    The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
    During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
    50.5K