介质素-10促进区多态性对反复流产的影响:一个病例控制方法
Neha Sudhir1, Mandeep Kaur1, Sukhjashanpreet Singh1
1Department of Human Genetics, Guru Nanak Dev University, Amritsar, Punjab, India.
Journal of human reproductive sciences
|August 7, 2023
概括
与印度的重复性流产 (RM) 相关,研究了介质素-10 (IL-10) 基因多态性. rs1800896多态 (GG基因型和G基因基因) 显示出与增加RM风险的显著关联.
科学领域:
- 生殖免疫学 生殖免疫学
- 人类遗传学 人类遗传学
- 分子生物学分子生物学
背景情况:
- 经常性流产 (RM) 影响0.8%-13.5%的女性,多因素导致,包括遗传,解剖,激素和免疫因素.
- 异常性RM约占所有病例的一半,强调需要进一步的病因学调查.
- 了解RM的遗传基础对于开发有针对性的诊断和治疗策略至关重要.
研究的目的:
- 调查印度人口中特定的Interleukin-10 (IL-10) 基因多态化与反复流产 (RM) 风险之间的关联.
- 在RM患者和健康对照中分析IL-10多态 rs1800872和rs1800896的基因型和等位基因频率.
- 在印度人口统计背景下提供基线遗传数据.
主要方法:
- 一项涉及RM的199名妇女和来自印度人口的215名健康对照的病例控制研究.
- 用基于聚合酶链反应 (PCR) 的方法进行了IL-10多态 rs1800872和rs1800896的基因型定型.
- 统计分析包括Student的t测试,Pearson的Hardy-Weinberg平衡的Chi-square测试,以及使用SPSS对等性,基因型和单元型频率的分析.
主要成果:
- 在RM病例和对照群之间 IL-10 rs1800872 多态之间没有观察到基因型或等位基因频率的显著差异 (P > 0.05).
- IL-10 rs1800896多态的GG基因型 (P = 0.007) 和G等位基因 (P = 0.003) 与反复流产的风险增加有显著关联.
- 对于rs1800896的主导和共同主导的遗传模型,发现了显著的差异,但哈普洛型分析没有揭示RM的任何特定风险哈普洛型.
结论:
- IL-10 rs1800896多态性,特别是GG基因型和G等位基因,与印度人口中反复流产的风险增加有关.
- 在这个队列中,IL-10的rss1800872多态性似乎不是RM的重要危险因素.
- 这项研究为印度RM的遗传易感性提供了新的见解,特别是在IL-10基因变异方面.
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