化对在正常和氧化脂质双层中嵌入的抗微生物piscidins的影响
1Department of Chemistry and Biochemistry, Old Dominion University Norfolk VA 23529 USA.
RSC chemical biology
|August 7, 2023
概括
在piscidins中的氨基终端Cu (ii) -和Ni (ii) -结合 (ATCUN) 基因的金属化增强了抗菌活性. 结合改变了动力学和膜相互作用,其依赖于序列的效应影响了金属的保护和有效性.
科学领域:
- 生物物理学的生物物理.
- 计算化学的计算化学
- 抗微生物是一种抗菌.
背景情况:
- 皮西丁是抗微生物,具有潜在的治疗应用.
- 在 piscidins 中的 N-终端氨基终端 Cu(ii) - 和 Ni(ii) - 结合 (ATCUN) 基因是金属化的目标.
- 化可能会调节piscidins的抗菌性质.
研究的目的:
- 研究 (II) 化对 piscidins 1 和 3 (P1 和 P3) 的结构和动态的影响.
- 探索金属化如何影响piscidins与脂质双层的相互作用.
- 了解序列在金属结合和抗菌活性中的作用.
主要方法:
- 用分子动力学模拟来研究自由和化P1和P3.
- 模拟使用现实的脂质双层模型 (POPC/POPG和POPC/POPG/aldo-PC) 进行.
- 分析的重点是结构动力学,N端电荷和双层内部的相互作用.
主要成果:
- (II) 结合减少了ATCUN图案的形态动态,并降低了N端电荷.
- 金属化piscidins嵌入更深入的双层,与的充电半螺旋和头组之间的相互作用.
- 在P1中观察到特定的相互作用 (cation-π,CH-π),为结合的金属提供了部分保护,而P3的序列则在固态上阻碍了这些相互作用.
结论:
- 皮西丁的尼基化改变了它们的膜相互作用和动态,可能增强了它们的抗微生物活性.
- 序列显著影响结合金属的稳定性和保护性相互作用,这表明调整抗菌疗效的机制.
- 金属化素可能会影响双层完整性,特别是在氧化脂的存在下.
更多相关视频
12:18Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
Published on: August 3, 2021
3.6K
11:04Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
Published on: September 7, 2019
9.2K
相关概念视频
01:23Antimicrobial Proteins
1.0K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
1.0K
01:28Antimicrobial Effectiveness
48
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
48
01:28Gene Regulation in Microbial Communities: Quorum Sensing
40
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
40
02:51Metal-Ligand Bonds
21.0K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
21.0K