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在结直肠腺癌中,COX-2和NUCB1的表达和相关性
Yuanyuan Zhang1, Gai Zhang2, Jinghua Zhong1
1Department of Oncology, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
PeerJ
|August 7, 2023
概括
环氧化原酶-2 (COX-2) 和核丁-1 (NUCB1) 在结直肠腺癌中都过度表达. 它们的表达水平与临床因素有显著的相关性和联系,这表明它们在结直肠癌的进展中起着作用.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 结肠直肠腺癌是全球重要的健康问题.
- 了解驱动结直肠癌的分子机制对于开发有效疗法至关重要.
- 循环氧化酶-2 (COX-2) 和核丁-1 (NUCB1) 在结直肠癌中的作用需要进一步阐明.
研究的目的:
- 研究结直肠腺癌中COX-2和NUCB1的表达模式.
- 确定COX-2和NUCB1在结直肠癌中的表达之间的相关性.
- 评估COX-2和NUCB1表达与临床病理特征和预后的关联.
主要方法:
- 使用STRING,UALCAN和人类蛋白质图谱 (HPA) 数据库进行生物信息分析.
- 免疫组织化学 (IHC) 评估56种结直肠腺癌和相邻组织样本中的蛋白质表达.
- 统计分析包括奇平方和斯皮尔曼相关性测试来评估关联.
主要成果:
- 生物信息分析表明,COX-2和NUCB1之间存在强烈联系.
- 在结肠直肠腺癌中,COX-2在基因和蛋白质水平上升调节,NUCB1在基因和蛋白质水平下降调节.
- 与相邻组织相比,IHC证实癌症组织中COX-2 (69.64%) 和NUCB1 (64.29%) 的表达显著更高.
- 无论是COX-2还是NUCB1表达都与临床阶段,分化程度和淋巴转移 (P <0.05) 有正相关.
- 在COX-2和NUCB1表达之间发现了显著的正相关性 (rs = 0.6312,P < 0.001).
结论:
- 在结直肠腺癌中,COX-2和NUCB1显著过度表达.
- 在结直肠癌中,COX-2和NUCB1表达之间存在显著的正相关性.
- 这两种分子的表达与不良的临床病理特征有关,表明它们作为生物标志物的潜力.
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